TY - JOUR
T1 - Proton relaxation times in bleomycin-induced lung injury
AU - Taylor, Caroline R.
AU - Dirk Sostman, H.
AU - Gore, John C.
AU - Walker Smith, G.
PY - 1987/8
Y1 - 1987/8
N2 - Pulmonary fibrosis and in flammation induced in mice by biweekly injection of low doses (20 mg/kg) of bleomycin chronically administered subcutaneously was associated with a significant increase in T2 values measured in vitro (54.6 ± 20.7 ms vs. 36.2 ± 3.8 ms in controls). T1 values, however, were not altered significantly (613 ± 124.6 ms vs. 666 ± 105.7 ms in controls). Lung water content did not change (79.1 ± 2.5% vs. 78.4 ± 0.98% in controls, NS). Water content to dry weight ratio in the control group (3.63 ± 0.212) did not differ significantly from that in the experimental group clinically treated with bleomycin (3.84 ± .618). However, morphometric and histologic analysis indicated that in 71% of the chronically treated mice, there was marked pulmonary fibrosis (mean area of involvement 3.5 ± 8.1% vs. 0.25 ± 0.15 in controls, P<.01). In addition, there was marked increase in pulmonary cellularity and infiltration by lymphocytes and macrophages (mean area of macrophage infiltration 8.71 ± 13.256% vs. 1.17 ± 1.404% in controls). The alteration in T2 in bleomycin damged lung occurred in the absence of change in overall water content. However, the change in cellularity indicates an alteration of water distribution between the cellular, intravascular, and interstitial components induced by the measurable fibrotic and inflammatory response to bleomycin.
AB - Pulmonary fibrosis and in flammation induced in mice by biweekly injection of low doses (20 mg/kg) of bleomycin chronically administered subcutaneously was associated with a significant increase in T2 values measured in vitro (54.6 ± 20.7 ms vs. 36.2 ± 3.8 ms in controls). T1 values, however, were not altered significantly (613 ± 124.6 ms vs. 666 ± 105.7 ms in controls). Lung water content did not change (79.1 ± 2.5% vs. 78.4 ± 0.98% in controls, NS). Water content to dry weight ratio in the control group (3.63 ± 0.212) did not differ significantly from that in the experimental group clinically treated with bleomycin (3.84 ± .618). However, morphometric and histologic analysis indicated that in 71% of the chronically treated mice, there was marked pulmonary fibrosis (mean area of involvement 3.5 ± 8.1% vs. 0.25 ± 0.15 in controls, P<.01). In addition, there was marked increase in pulmonary cellularity and infiltration by lymphocytes and macrophages (mean area of macrophage infiltration 8.71 ± 13.256% vs. 1.17 ± 1.404% in controls). The alteration in T2 in bleomycin damged lung occurred in the absence of change in overall water content. However, the change in cellularity indicates an alteration of water distribution between the cellular, intravascular, and interstitial components induced by the measurable fibrotic and inflammatory response to bleomycin.
KW - Bleomycin
KW - Lung fibrosis
KW - Nuclear magnetic resonance
KW - Proton relaxation times
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U2 - 10.1097/00004424-198708000-00001
DO - 10.1097/00004424-198708000-00001
M3 - Article
C2 - 2444553
AN - SCOPUS:0023629661
VL - 22
SP - 621
EP - 626
JO - Investigative Radiology
JF - Investigative Radiology
SN - 0020-9996
IS - 8
ER -