@article{ddd16789ac7d4adfbbcf7d8a9797afcf,
title = "Proton magnetic resonance spectroscopy characterization of Rathke{\textquoteright}s cleft cysts (RCCs): Relevance to the differential diagnosis of pituitary adenomas and RCCs",
abstract = "Background: Rathke{\textquoteright}s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.",
keywords = "Cholesterol, Glycosaminoglycans, Magnetic resonance spectroscopy, Pituitary adenoma, Rathke{\textquoteright}s cleft cyst",
author = "Ijare, {Omkar B.} and Sharpe, {Martyn A.} and Baskin, {David S.} and Kumar Pichumani",
note = "Funding Information: Funding: This study was supported by the Donna and Kenneth R. Peak Foundation, The Kenneth R. Peak Brain and Pituitary Tumor Treatment Center at Houston Methodist Hospital, The Houston Methodist Foundation, The Taub Foundation, The Pauline Sterne Wolff Foundation, The Veralan Foundation, The Marilee A. and Gary M. Schwarz Foundation, The John S. Dunn Foundation and The McKone Family Foundation. Funding Information: This study was supported by the Donna and Kenneth R. Peak Foundation, The Kenneth R. Peak Brain and Pituitary Tumor Treatment Center at Houston Methodist Hospital, The Houston Methodist Foundation, The Taub Foundation, The Pauline Sterne Wolff Foundation, The Veralan Foundation, The Marilee A. and Gary M. Schwarz Foundation, The John S. Dunn Foundation and The McKone Family Foundation. sWe thank all the patients who participated in this study and the members of Neurosurgery team of D.S.B. at The Houston Methodist Hospital for obtaining RCCs and pituitary adenoma specimens during surgery. We also thank the NMR and Drug Metabolism Core at the Baylor College of Medicine, Houston for allowing us to collect the NMR data at their Facility. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = feb,
doi = "10.3390/cancers12020360",
language = "English (US)",
volume = "12",
journal = "Cancers",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",
}