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Protocol for single-use Ambu aScope-based minimally invasive bronchoalveolar lavage collection in pigs

Piyush Baindara, Dinata Roy, Addison K. Byrne, Jonathan A. Green

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we present a protocol for collecting bronchoalveolar lavage (BAL) samples from live pigs using a single-use Ambu aScope. We describe the steps for animal preparation, BAL collection, and sample handling, including downstream analysis, to ensure consistent, high-quality results. This approach applies to a wide range of respiratory research, including the evaluation of inflammatory responses and the testing of new therapies in clinically relevant pig models.

Original languageEnglish (US)
Article number104363
Pages (from-to)104363
JournalSTAR Protocols
Volume7
Issue number1
DOIs
StatePublished - Mar 20 2026

Keywords

  • Animals
  • Bronchoalveolar Lavage/methods
  • Swine
  • Bronchoalveolar Lavage Fluid/chemistry
  • Specimen Handling/methods

ASJC Scopus subject areas

  • General Neuroscience
  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology

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