Proteomic profiling of a biomimetic drug delivery platform

Claudia Corbo, Alessandro Parodi, Michael Evangelopoulos, David A. Engler, Risë K. Matsunami, Anthony C. Engler, Roberto Molinaro, Shilpa Scaria, Francesco Salvatore, Ennio Tasciotti

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Current delivery platforms are typically designed for prolonged circulation that favors superior accumulation of the payload in the targeted tissue. The design of efficient surface modifications determines both a longer circulation time and targeting abilities of particles. The optimization of synthesis protocols to efficiently combine targeting molecules and elements that allow for an increased circulation time can be challenging and almost impossible when several functional elements are needed. On the other hand, in the last decade, the development of bioinspired technologies was proposed as a new approach with which to increase particle safety, biocompatibility and targeting, while maintaining the synthesis protocols simple and reproducible. Recently, we developed a new drug delivery system inspired by the biology of immune cells called leukolike vector (LLV) and formed by a nanoporous silicon core and a shell derived from the leucocyte cell membrane. The goal of this study is to investigate the protein content of the LLV. Here we report the proteomic profiling of the LLV and demonstrate that our approach can be used to modify the surface of synthetic particles with more than 150 leukocyte membraneassociated proteins that determine particle safety, circulation time and targeting abilities towards inflamed endothelium.

Original languageEnglish (US)
Article numberA014
Pages (from-to)1540-1547
Number of pages8
JournalCurrent Drug Targets
Volume16
Issue number13
DOIs
StatePublished - 2015

Keywords

  • Bio-mimetic camouflage
  • Drug delivery
  • Leukocyte
  • Leukolike vector
  • Membrane
  • Nanoparticles
  • Nanotechnology
  • Proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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