@article{afd6780b870346b1a9eaaa42a8d6578f,
title = "Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes",
abstract = "Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (PXD002319-PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].",
keywords = "Biochemical, Fractionation, Metazoa, Protein complexes, Proteomics",
author = "Sadhna Phanse and Cuihong Wan and Blake Borgeson and Fan Tu and Kevin Drew and Greg Clark and Xuejian Xiong and Olga Kagan and Julian Kwan and Alexandr Bezginov and Kyle Chessman and Swati Pal and Graham Cromar and Ophelia Papoulas and Zuyao Ni and Boutz, {Daniel R.} and Snejana Stoilova and Havugimana, {Pierre C.} and Xinghua Guo and Malty, {Ramy H.} and Mihail Sarov and Jack Greenblatt and Mohan Babu and Derry, {W. Brent} and Tillier, {Elisabeth R.} and Wallingford, {John B.} and John Parkinson and Marcotte, {Edward M.} and Andrew Emili",
note = "Funding Information: We thank T. Kwon (University of Texas) for X. laevis gene models, and K. Foltz (University of California, Santa Barbara), A. Brehm (Philipps-University Marburg), P. Paddison (Fred Hutchinson Cancer Research Center), J. Smith (Woods Hole Marine Biological Laboratory), P. Zandstra and J. Moffat (University of Toronto) for providing biological specimens and reagents. We thank members of the Emili and Marcotte laboratories for assistance and guidance, and SciNet (University of Toronto) and the Texas Advanced Computing Center (University of Texas) for high-performance computing resources. This work was supported by grants from the CIHR, NSERC, ORF and the CFI to A.E., from the CIHR and Heart and Stroke to J. P., from the NIH (F32GM112495) to K.D., and from the NIH, NSF, CPRIT, and Welch Foundation (F-1515) to E.M.M. Publisher Copyright: {\textcopyright} 2016 Published by Elsevier Inc.",
year = "2016",
month = mar,
day = "1",
doi = "10.1016/j.dib.2015.11.062",
language = "English (US)",
volume = "6",
pages = "715--721",
journal = "Data in Brief",
issn = "2352-3409",
publisher = "Elsevier BV",
}