Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells

Ying Zhou, Longhou Fang, Dan Du, Wenchao Zhou, Xiujing Feng, Jiwu Chen, Zhe Zhang, Zhengjun Chen

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The evolutionarily conserved cell polarity protein Par3, a scaffold-like PDZreontaining protein, plays a critical role in the establishment and maintenance of epithelial cell polarity. Although the role of Par3 in establishing cell polarity in epithelial cells has been intensively explored, the function of Par3 in hematopoietic cells remains elusive. To address this issue, we generated GST-fusion proteins of Par3 PDZ domains. By combiningthe GST-pull-down approach with liquid chromatography-tandem mass spectrometry, we identified 10 potential novel binding proteins of PDZ domains of Par3 in Jurkat cells (a T-cell line). The interaction of Par3 with three proteins - nuclear transport protein importin-α4 and proteasome activators PA28β and PA28γ - was confirmed using in vitro binding assay, co-immunoprecipitation assay and immunofluorescence microscopy. Our results have the potential to uncover novel functions of the cell polarity protein Par3 in blood cells.

Original languageEnglish (US)
Pages (from-to)729-739
Number of pages11
JournalActa Biochimica et Biophysica Sinica
Volume40
Issue number8
DOIs
StatePublished - Aug 2008

Keywords

  • Cell polarity
  • Importin-α4
  • PA28β
  • PA28γ
  • Par3
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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