Proteins and cells on PEG immobilized silicon surfaces

Miqin Zhang; Tejal Desai; Mauro Ferrari

doi:10.1016/S0142-9612(98)00026-X

Proteins and cells on PEG immobilized silicon surfaces

Miqin Zhang, Tejal Desai, Mauro Ferrari

Research output: Contribution to journal › Article › peer-review

381 Scopus citations

Abstract

Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.

Original language	English (US)
Pages (from-to)	953-960
Number of pages	8
Journal	Biomaterials
Volume	19
Issue number	10
DOIs	https://doi.org/10.1016/S0142-9612(98)00026-X
State	Published - May 1998
Externally published	Yes

Keywords

Cellular interaction
Ellipsometry
PEG immobilization
Protein adsorption
Silicon

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

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10.1016/S0142-9612(98)00026-X

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title = "Proteins and cells on PEG immobilized silicon surfaces",

abstract = "Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.",

keywords = "Cellular interaction, Ellipsometry, PEG immobilization, Protein adsorption, Silicon",

author = "Miqin Zhang and Tejal Desai and Mauro Ferrari",

year = "1998",

month = may,

doi = "10.1016/S0142-9612(98)00026-X",

language = "English (US)",

volume = "19",

pages = "953--960",

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T1 - Proteins and cells on PEG immobilized silicon surfaces

AU - Zhang, Miqin

AU - Desai, Tejal

AU - Ferrari, Mauro

PY - 1998/5

Y1 - 1998/5

N2 - Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.

AB - Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.

KW - Cellular interaction

KW - Ellipsometry

KW - PEG immobilization

KW - Protein adsorption

KW - Silicon

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JO - Biomaterials

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Proteins and cells on PEG immobilized silicon surfaces

Abstract

Keywords

ASJC Scopus subject areas

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