TY - JOUR
T1 - Protein phosphatase 2A regulatory subunits are starting to reveal their functions in plant metabolism and development
AU - Jonassen, Else Müller
AU - Heidari, Behzad
AU - Nemie-Feyissa, Dugassa
AU - Matre, Polina
AU - Lillo, Cathrine
PY - 2011/8
Y1 - 2011/8
N2 - Canonical protein phosphatase 2A (PP2A) consists of a catalytic subunit (C), a scaffolding subunit (A), and a regulatory subunit (B). The B subunits are believed to confer substrate specificity and cellular localization to the PP2A complex, and are generally divided into three non-related families in plants, i.e., B55, B' and B''. The two Arabidopsis B55 subunits (α and β) interact with nitrate reductase (NR) in the bimolecular fluorescence complementation assay in planta, and are necessary for rapid activation of NR. Interestingly, knockout of all four B55 alleles is probably lethal, because a homozygous double knockout (pp2a-b55αβ) could not be found. The B55 subunits, therefore, appear to have essential functions that cannot be replaced by other regulatory B subunits. A double mutant (pp2a-b'αβ) of two close B' homologs show severely impaired fertility, pointing to the essential role also of B' subunits in plant development.
AB - Canonical protein phosphatase 2A (PP2A) consists of a catalytic subunit (C), a scaffolding subunit (A), and a regulatory subunit (B). The B subunits are believed to confer substrate specificity and cellular localization to the PP2A complex, and are generally divided into three non-related families in plants, i.e., B55, B' and B''. The two Arabidopsis B55 subunits (α and β) interact with nitrate reductase (NR) in the bimolecular fluorescence complementation assay in planta, and are necessary for rapid activation of NR. Interestingly, knockout of all four B55 alleles is probably lethal, because a homozygous double knockout (pp2a-b55αβ) could not be found. The B55 subunits, therefore, appear to have essential functions that cannot be replaced by other regulatory B subunits. A double mutant (pp2a-b'αβ) of two close B' homologs show severely impaired fertility, pointing to the essential role also of B' subunits in plant development.
KW - Arabidopsis
KW - Brassinosteroids
KW - Light activation
KW - Nitrate reductase
KW - PP2A
KW - Seeds
KW - Siliques
UR - http://www.scopus.com/inward/record.url?scp=79961187351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961187351&partnerID=8YFLogxK
U2 - 10.4161/psb.6.8.16180
DO - 10.4161/psb.6.8.16180
M3 - Article
C2 - 21758015
AN - SCOPUS:79961187351
SN - 1559-2316
VL - 6
SP - 1216
EP - 1218
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 8
ER -