Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

Zrinka Bulj, Serena Duchi, Alessandro Bevilacqua, Alessandro Gherardi, Barbara Dozza, Filippo Piccinini, Giulia Adalgisa Mariani, Enrico Lucarelli, Sandro Giannini, Davide Donati, Sandra Marmiroli

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.

Original languageEnglish (US)
Pages (from-to)118-126
Number of pages9
JournalInternational Journal of Oncology
Volume42
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Cell migration
  • Mesenchymal stem cells
  • Phosphoinositide-3 kinase/protein kinase B pathway
  • Protein kinase B Inhibitors
  • Wound healing

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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