Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner

Xiaju Cheng, Xin Tian, Anqing Wu, Jianxiang Li, Jian Tian, Yu Chong, Zhifang Chai, Yuliang Zhao, Chunying Chen, Cuicui Ge

Research output: Contribution to journalArticlepeer-review

159 Scopus citations

Abstract

The interaction at nanobio is a critical issue in designing safe nanomaterials for biomedical applications. Recent studies have reported that it is nanoparticle-protein corona rather than bare nanoparticle that determines the nanoparticle-cell interactions, including endocytic pathway and biological responses. Here, we demonstrate the effects of protein corona on cellular uptake of different sized gold nanoparticles in different cell lines. The experimental results show that protein corona significantly decreases the internalization of Au NPs in a particle size- and cell type-dependent manner. Protein corona exhibits much more significant inhibition on the uptake of large-sized Au NPs by phagocytic cell than that of small-sized Au NPs by nonphagocytic cell. The endocytosis experiment indicates that different endocytic pathways might be responsible for the differential roles of protein corona in the interaction of different sized Au NPs with different cell lines. Our findings can provide useful information for rational design of nanomaterials in biomedical application.

Original languageEnglish (US)
Pages (from-to)20568-20575
Number of pages8
JournalACS Applied Materials and Interfaces
Volume7
Issue number37
DOIs
StatePublished - Sep 23 2015

Keywords

  • cell line
  • endocytic pathways
  • gold nanoparticle
  • protein corona
  • size
  • uptake level

ASJC Scopus subject areas

  • Materials Science(all)

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