Protease expression in dedifferentiated parosteal osteosarcoma

Carsten Haeckel, Alberto Ayala, Kathrin Radig, A. Kevin Raymond, Albert Roessner, Bogdan Czerniak

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Background. - Parosteal osteosarcoma with dedifferentiation provides a useful model to study tumor progression from an indolent locally aggressive neoplasm to highly lethal metastasizing malignancy. Up-regulation of the proteolytic enzymes participating in stromal degradation is known to promote invasive growth and metastasis of several human and experimental tumors. Methods. - The expression patterns of urokinasase plasminogen activator (u- PA), its cell-surface receptor (u-PAR), and cathepsin B were analyzed by immunohistochemical techniques in 11 cases of parosteal osteosarcoma and in 4 cases of dedifferentiated parosteal osteosarcoma. Results. - Both enzymes and the receptor were coexpressed in most tumor cells of parosteal and dedifferentiated parosteal osteosarcoma. Their expression was strikingly enhanced in the dedifferentiated high-grade component of the tumors. Tumor cells involved in bone production (ie, those adjacent to tumor produced bone trabeculae) exhibited equally strong expression of u-PA, u-PAR, and cathepsin B, regardless of their histologic grade. Expression of u-PA, u-PAR, and cathepsin B was undetectable in the 'normalized' cells embedded in the well- developed tumor bone trabeculae. Conclusion. - These data indicate that u-PA and its interacting molecules, such as u-PAR and cathepsin B, may have some contributory effects on the metastatic potential of tumor cells in dedifferentiated parosteal osteosarcoma.

Original languageEnglish (US)
Pages (from-to)213-221
Number of pages9
JournalArchives of Pathology and Laboratory Medicine
Volume123
Issue number3
StatePublished - Mar 22 1999

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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