Abstract
OBJECTIVE: To investigate the arrest effect of diallyl disulfide (DADS) in the cell cycle of human nasopharyngeal carcinoma SW480 cell line and its molecular mechanism. METHODS: The growth inhibition effect of different concentrations of DADS (50 μg/mL) on SW480 cell line was measured by MTT assay and cell counting. Apoptosis rate was analyzed by flow cytometry. CK19 mRNA level was detected by RT-PCR. The expression of CK19 was determined by Western blotting analysis. RESULTS: The MTT assay showed that DADS inhibited growth of SW480 cells significantly with dose dependent. There was a significant difference between the treated and controlled cases (P<0.05). Cell counting results showed that average doubling time increased with the dose increase of DADS, compared with normal cultured SW480 cells (P< 0.05). Flow cytometry analysis revealed that SW480 cells treated with 50 μg/mL DADS increased the apoptosis rate. CK19 mRNA was decreased in SW480 treated with 50 μg/mL DADS using RT-PCR. CK19 was decreased significantly in SW480 treated with 50 μg/mL DADS by using Western blotting. The differences were more than two timesc. CONCLUSION: The anti-proliferation property of DADS in cultured human colon carcinoma SW480 cell line may relate to its ability of increasing the apoptosis rate through down-regulation of CK19.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 419-423 |
| Number of pages | 5 |
| Journal | Chinese Journal of Cancer Prevention and Treatment |
| Volume | 16 |
| Issue number | 6 |
| State | Published - Mar 2009 |
Keywords
- Cell line, tumor
- Colonic neoplasms
- Disulfides
- Keratin
ASJC Scopus subject areas
- Oncology