Prokaryotic expression, purification, and production of glutathione S-transferase-tagged neural stem cell specific peptides from phage display screening

Lei Jin, Wenxiu Zhao, Ma Lan

Research output: Contribution to journalArticle

Abstract

Combining stem cell with phage display to discover novel biomarkers is a new field in stem cell studies. Novel peptides obtained through phage display panning have been functionally identified and involved into initial applications as cell culture support or cell label. In the present study, we designed a glutathione S-transferase (GST)-tagged peptide complex to construct an easy and efficient prokaryotic expression and purification platform for peptides obtained from phage display panning. The purified GST-peptide protein could specifically bind to neural stem (NS) cells and could be flexibly used for GST pull down assay or NS cell labeling in future studies. The results of the present study would be useful for cell labeling and future investigations of special receptors on stem cell surface.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalProtein and Peptide Letters
Volume20
Issue number2
DOIs
StatePublished - Jan 1 2013

Keywords

  • Glutathione-S-transferase-tagged
  • Neural stem cell
  • Peptide
  • Phage display
  • Prokaryotic expression

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology

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