TY - JOUR
T1 - Profiling gene expression in human placentae of different gestational ages
T2 - An OPRU network and UW SCOR study
AU - Mikheev, Andrei M.
AU - Nabekura, Tomohiro
AU - Kaddoumi, Amal
AU - Bammler, Theo K.
AU - Govindarajan, Rajgopal
AU - Hebert, Mary F.
AU - Unadkat, Jashvant D.
PY - 2008/9
Y1 - 2008/9
N2 - We used the whole-genome approach to identify major functional categories of genes whose expression depends on gestational age. Using microarray analysis, we compared gene expression profiles in the villous tissues of first (45-59 days) and second trimester (109-115 days) placentae with C-section term placentae. We found that in first trimester placentae, genes related to cell cycle, DNA, amino acids, and carbohydrate metabolism were significantly overrepresented, while genes related to signal transduction were underrepresented. Among genes involved in organism defense, we identified genes involved in chemical response, metabolism, and transport. Analysis of signal transduction pathways suggested, and subsequently confirmed independently, that the Wnt pathway was changed with gestational age leading to inhibition of β-catenin protein expression. Our study will serve as a reference database to gain insight into the regulation of gene expression in the developing placentae and to compare with gene expression in placentae from complicated pregnancies.
AB - We used the whole-genome approach to identify major functional categories of genes whose expression depends on gestational age. Using microarray analysis, we compared gene expression profiles in the villous tissues of first (45-59 days) and second trimester (109-115 days) placentae with C-section term placentae. We found that in first trimester placentae, genes related to cell cycle, DNA, amino acids, and carbohydrate metabolism were significantly overrepresented, while genes related to signal transduction were underrepresented. Among genes involved in organism defense, we identified genes involved in chemical response, metabolism, and transport. Analysis of signal transduction pathways suggested, and subsequently confirmed independently, that the Wnt pathway was changed with gestational age leading to inhibition of β-catenin protein expression. Our study will serve as a reference database to gain insight into the regulation of gene expression in the developing placentae and to compare with gene expression in placentae from complicated pregnancies.
KW - Gestational age
KW - Metabolizing enzymes
KW - Microarray
KW - Placenta
KW - Wnt pathway
KW - Xenobiotic transporters
UR - http://www.scopus.com/inward/record.url?scp=57449110735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57449110735&partnerID=8YFLogxK
U2 - 10.1177/1933719108322425
DO - 10.1177/1933719108322425
M3 - Article
C2 - 19050320
AN - SCOPUS:57449110735
SN - 1933-7191
VL - 15
SP - 866
EP - 877
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 9
ER -