Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein-Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant

Uluhan Sili, Ann M. Leen, Juan F. Vera, Adrian P. Gee, Helen Huls, Helen E. Heslop, Catherine M. Bollard, Cliona M. Rooney

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Infections with a range of common community viruses remain a major cause of mortality and morbidity after allogeneic hematopoietic stem cell transplantation. T cells specific for cytomegalovirus (CMV), Epstein-Barr virus (EBV) and adenoviruses can safely prevent and infections with these three most common culprits, but the manufacture of individual T cell lines for each virus would be prohibitive in terms of time and cost. We have demonstrated that T cells specific for all three viruses can be manufactured in a single culture using monocytes and EBV-transformed B lymphoblastoid cell lines (LCLs), both transduced with an adenovirus vector expressing pp65 of CMV, as antigen-presenting cells. Trivirus-specific T cell lines produced from healthy stem cell donors could prevent and treat infections with all three viruses, not only in the designated recipient, but in unrelated, partially-HLA-matched third party recipients. We now provide the details and logistics of T cell manufacture.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalCytotherapy
Volume14
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • adenovirus
  • CMV
  • Cytotoxic T cells (CTLs)
  • EBV
  • HSCT
  • Immunotherapy
  • viral infections

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Molecular Medicine

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