Production of Genetically Modified Epstein-Barr Virus-Specific Cytotoxic T Cells for Adoptive Transfer to Patients at High Risk of EBV-Associated Lymphoproliferative Disease

Colton A. Smith, Catherine Y.C. Ng, Helen E. Heslop, Martha S. Holladay, Stacye Richardson, E. Victoria Turner, Susan K. Loftin, Congfen Li, Malcolm K. Brenner, Cliona M. Rooney

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

EBV-induced lymphoproliferative disease (EBV-LPD) is a disorder most commonly associated with the immunocompromise that follows allogeneic organ transplantation. In patients receiving T cell-depleted bone marrow from HLA- mismatched or HLA-matched unrelated donors, the incidence of EBV-LPD is particularly high, ranging from 5 to 30%. Administration of EBV-specific cytotoxic T lymphocytes may be one means of preventing and treating this disease. We now describe a method that allows the routine and timely preparation of large numbers of such cells to allow their safe administration to bone marrow transplant recipients. We also describe how these cells may be genetically marked before infusion, to determine their fate and disposition in vivo.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalJournal of Hematotherapy and Stem Cell Research
Volume4
Issue number2
DOIs
StatePublished - Apr 1995

ASJC Scopus subject areas

  • Immunology
  • Hematology

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