TY - JOUR
T1 - Production of functional S-II in Bombyx mori cells
AU - Hirashima, Shoji
AU - Horikoshi, Nobuo
AU - Sekimizu, Kazuhisa
AU - Natori, Shunji
N1 - Funding Information:
We wish to thank Drs. Y. Saeki and M. Furusawa (Daiichi Seiyaku Co. Ltd.) for providing the baculovirus expression system and for helping us to construct recombinant viruses. This study was supported by a Grant-in-Aid for Scientific Research on Priority Areas of Transcription Factors from the Ministry of Education, Science and Culture of Japan and a grant from the Japan Health Science Foundation.
PY - 1989/5/15
Y1 - 1989/5/15
N2 - A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.
AB - A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 μg of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.
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U2 - 10.1016/S0006-291X(89)80115-9
DO - 10.1016/S0006-291X(89)80115-9
M3 - Article
C2 - 2730640
AN - SCOPUS:0024970462
SN - 0006-291X
VL - 160
SP - 1093
EP - 1099
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -