Production of cholesteryl ester-rich, anisotropic inclusions by mammalian cells in culture

George H. Rothblat; Joel M. Rosen; William Insull; Annie O. Yau; Donald M. Small

doi:10.1016/0014-4800(77)90059-4

Production of cholesteryl ester-rich, anisotropic inclusions by mammalian cells in culture

George H. Rothblat, Joel M. Rosen, William Insull, Annie O. Yau, Donald M. Small

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22 Scopus citations

Abstract

Growth of Fu5AH hepatoma cells in tissue culture medium supplemented with hyperlipemic rabbit serum results in the accumulation of cholesteryl ester-rich, anisotropic inclusions similar to the inclusions which accumulate in atherosclerotic aorta of man and animals. Scanning electron microscopic studies of whole cells and isolated inclusions have been performed. The chemical composition of cellular lipid inclusions has been determined and can be related to the observed anisotropicisotropic phase transition temperatures.

Original language	English (US)
Pages (from-to)	318-324
Number of pages	7
Journal	Experimental and Molecular Pathology
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/0014-4800(77)90059-4
State	Published - Apr 1977

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Clinical Biochemistry

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abstract = "Growth of Fu5AH hepatoma cells in tissue culture medium supplemented with hyperlipemic rabbit serum results in the accumulation of cholesteryl ester-rich, anisotropic inclusions similar to the inclusions which accumulate in atherosclerotic aorta of man and animals. Scanning electron microscopic studies of whole cells and isolated inclusions have been performed. The chemical composition of cellular lipid inclusions has been determined and can be related to the observed anisotropicisotropic phase transition temperatures.",

author = "Rothblat, {George H.} and Rosen, {Joel M.} and William Insull and Yau, {Annie O.} and Small, {Donald M.}",

year = "1977",

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T1 - Production of cholesteryl ester-rich, anisotropic inclusions by mammalian cells in culture

AU - Rothblat, George H.

AU - Rosen, Joel M.

AU - Insull, William

AU - Yau, Annie O.

AU - Small, Donald M.

PY - 1977/4

Y1 - 1977/4

N2 - Growth of Fu5AH hepatoma cells in tissue culture medium supplemented with hyperlipemic rabbit serum results in the accumulation of cholesteryl ester-rich, anisotropic inclusions similar to the inclusions which accumulate in atherosclerotic aorta of man and animals. Scanning electron microscopic studies of whole cells and isolated inclusions have been performed. The chemical composition of cellular lipid inclusions has been determined and can be related to the observed anisotropicisotropic phase transition temperatures.

AB - Growth of Fu5AH hepatoma cells in tissue culture medium supplemented with hyperlipemic rabbit serum results in the accumulation of cholesteryl ester-rich, anisotropic inclusions similar to the inclusions which accumulate in atherosclerotic aorta of man and animals. Scanning electron microscopic studies of whole cells and isolated inclusions have been performed. The chemical composition of cellular lipid inclusions has been determined and can be related to the observed anisotropicisotropic phase transition temperatures.

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ER -

Production of cholesteryl ester-rich, anisotropic inclusions by mammalian cells in culture

Abstract

ASJC Scopus subject areas

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