Production of cholesteryl ester-rich, anisotropic inclusions by mammalian cells in culture

George H. Rothblat, Joel M. Rosen, William Insull, Annie O. Yau, Donald M. Small

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Growth of Fu5AH hepatoma cells in tissue culture medium supplemented with hyperlipemic rabbit serum results in the accumulation of cholesteryl ester-rich, anisotropic inclusions similar to the inclusions which accumulate in atherosclerotic aorta of man and animals. Scanning electron microscopic studies of whole cells and isolated inclusions have been performed. The chemical composition of cellular lipid inclusions has been determined and can be related to the observed anisotropicisotropic phase transition temperatures.

Original languageEnglish (US)
Pages (from-to)318-324
Number of pages7
JournalExperimental and Molecular Pathology
Volume26
Issue number2
DOIs
StatePublished - Apr 1977

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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