Production of antibody by human B cells under serum-free conditions

J. Farrant; C. A. Newton; M. E. North; C. Weyman; Malcolm Brenner

doi:10.1016/0022-1759(84)90133-9

Production of antibody by human B cells under serum-free conditions

J. Farrant, C. A. Newton, M. E. North, C. Weyman, Malcolm Brenner

Research output: Contribution to journal › Article

21 Scopus citations

Abstract

A method is described for growing human B cells in 20 μl hanging drops in Terasaki plates under serum-free conditions. B cell proliferation and differentiation has a critical dependence for added soybean lipid, while T cell proliferation does not. In this medium, pokeweed mitogen stimulation of separated human B cells induces high levels of immunoglobulin in a T dependent manner. Cells from donors vaccinated with tetanus toxoid and shown to be responders by a conventional culture system, produce high levels of IgG anti-tetanus antibody after antigen stimulation in these serum-free microcultures. The serum free culture system has the novel features of high sensitivity to dose of mitogen or antigen, low background responses and high antibody production with low cell numbers.

Original language	English (US)
Pages (from-to)	25-34
Number of pages	10
Journal	Journal of Immunological Methods
Volume	68
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(84)90133-9
State	Published - Mar 30 1984

Keywords

antibody production in vitro
serum-free lymphocyte culture
soybean lipid

ASJC Scopus subject areas

Immunology
Biotechnology

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title = "Production of antibody by human B cells under serum-free conditions",

abstract = "A method is described for growing human B cells in 20 μl hanging drops in Terasaki plates under serum-free conditions. B cell proliferation and differentiation has a critical dependence for added soybean lipid, while T cell proliferation does not. In this medium, pokeweed mitogen stimulation of separated human B cells induces high levels of immunoglobulin in a T dependent manner. Cells from donors vaccinated with tetanus toxoid and shown to be responders by a conventional culture system, produce high levels of IgG anti-tetanus antibody after antigen stimulation in these serum-free microcultures. The serum free culture system has the novel features of high sensitivity to dose of mitogen or antigen, low background responses and high antibody production with low cell numbers.",

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T1 - Production of antibody by human B cells under serum-free conditions

AU - Farrant, J.

AU - Newton, C. A.

AU - North, M. E.

AU - Weyman, C.

AU - Brenner, Malcolm

PY - 1984/3/30

Y1 - 1984/3/30

N2 - A method is described for growing human B cells in 20 μl hanging drops in Terasaki plates under serum-free conditions. B cell proliferation and differentiation has a critical dependence for added soybean lipid, while T cell proliferation does not. In this medium, pokeweed mitogen stimulation of separated human B cells induces high levels of immunoglobulin in a T dependent manner. Cells from donors vaccinated with tetanus toxoid and shown to be responders by a conventional culture system, produce high levels of IgG anti-tetanus antibody after antigen stimulation in these serum-free microcultures. The serum free culture system has the novel features of high sensitivity to dose of mitogen or antigen, low background responses and high antibody production with low cell numbers.

AB - A method is described for growing human B cells in 20 μl hanging drops in Terasaki plates under serum-free conditions. B cell proliferation and differentiation has a critical dependence for added soybean lipid, while T cell proliferation does not. In this medium, pokeweed mitogen stimulation of separated human B cells induces high levels of immunoglobulin in a T dependent manner. Cells from donors vaccinated with tetanus toxoid and shown to be responders by a conventional culture system, produce high levels of IgG anti-tetanus antibody after antigen stimulation in these serum-free microcultures. The serum free culture system has the novel features of high sensitivity to dose of mitogen or antigen, low background responses and high antibody production with low cell numbers.

KW - antibody production in vitro

KW - serum-free lymphocyte culture

KW - soybean lipid

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