Production of antibody by human B cells under serum-free conditions

J. Farrant, C. A. Newton, M. E. North, C. Weyman, Malcolm Brenner

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A method is described for growing human B cells in 20 μl hanging drops in Terasaki plates under serum-free conditions. B cell proliferation and differentiation has a critical dependence for added soybean lipid, while T cell proliferation does not. In this medium, pokeweed mitogen stimulation of separated human B cells induces high levels of immunoglobulin in a T dependent manner. Cells from donors vaccinated with tetanus toxoid and shown to be responders by a conventional culture system, produce high levels of IgG anti-tetanus antibody after antigen stimulation in these serum-free microcultures. The serum free culture system has the novel features of high sensitivity to dose of mitogen or antigen, low background responses and high antibody production with low cell numbers.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalJournal of Immunological Methods
Volume68
Issue number1-2
DOIs
StatePublished - Mar 30 1984

Keywords

  • antibody production in vitro
  • serum-free lymphocyte culture
  • soybean lipid

ASJC Scopus subject areas

  • Immunology
  • Biotechnology

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