TY - JOUR
T1 - Probing Adsorption Behaviors of BSA onto Chiral Surfaces of Nanoparticles
AU - Wang, Xinyi
AU - Wang, Xiaofeng
AU - Wang, Mingzhe
AU - Zhang, Di
AU - Yang, Qi
AU - Liu, Tao
AU - Lei, Rong
AU - Zhu, Shuifang
AU - Zhao, Yuliang
AU - Chen, Chunying
PY - 2018/4/19
Y1 - 2018/4/19
N2 - Chiral properties of nanoscale materials are of importance as they dominate interactions with proteins in physiological environments; however, they have rarely been investigated. In this study, a systematic investigation is conducted for the adsorption behaviors of bovine serum albumin (BSA) onto the chiral surfaces of gold nanoparticles (AuNPs), involving multiple techniques and molecular dynamic (MD) simulation. The adsorption of BSA onto both L- and D-chiral surfaces of AuNPs shows discernible differences involving thermodynamics, adsorption orientation, exposed charges, and affinity. As a powerful supplement, MD simulation provides a molecular-level understanding of protein adsorption onto nanochiral surfaces. Salt bridge interaction is proposed as a major driving force at protein–nanochiral interface interaction. The spatial distribution features of functional groups (COO−, NH3 +, and CH3) of chiral molecules on the nanosurface play a key role in the formation and location of salt bridges, which determine the BSA adsorption orientation and binding strength to chiral surfaces. Sequentially, BSA corona coated on nanochiral surfaces affects their uptake by cells. The results enhance the understanding of protein corona, which are important for biological effects of nanochirality in living organisms.
AB - Chiral properties of nanoscale materials are of importance as they dominate interactions with proteins in physiological environments; however, they have rarely been investigated. In this study, a systematic investigation is conducted for the adsorption behaviors of bovine serum albumin (BSA) onto the chiral surfaces of gold nanoparticles (AuNPs), involving multiple techniques and molecular dynamic (MD) simulation. The adsorption of BSA onto both L- and D-chiral surfaces of AuNPs shows discernible differences involving thermodynamics, adsorption orientation, exposed charges, and affinity. As a powerful supplement, MD simulation provides a molecular-level understanding of protein adsorption onto nanochiral surfaces. Salt bridge interaction is proposed as a major driving force at protein–nanochiral interface interaction. The spatial distribution features of functional groups (COO−, NH3 +, and CH3) of chiral molecules on the nanosurface play a key role in the formation and location of salt bridges, which determine the BSA adsorption orientation and binding strength to chiral surfaces. Sequentially, BSA corona coated on nanochiral surfaces affects their uptake by cells. The results enhance the understanding of protein corona, which are important for biological effects of nanochirality in living organisms.
KW - chiral surfaces
KW - molecular dynamic simulation
KW - nanoparticles
KW - protein adsorption behavior
UR - http://www.scopus.com/inward/record.url?scp=85045838806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045838806&partnerID=8YFLogxK
U2 - 10.1002/smll.201703982
DO - 10.1002/smll.201703982
M3 - Article
C2 - 29573549
AN - SCOPUS:85045838806
VL - 14
JO - Small
JF - Small
SN - 1613-6810
IS - 16
M1 - 1703982
ER -