Primary cell-mediated lympholysis response to a maternally transmitted antigen

Roger Smith Iii; David P. Huston; Robert R. Rich

doi:10.1084/jem.156.6.1866

Primary cell-mediated lympholysis response to a maternally transmitted antigen

Roger Smith Iii, David P. Huston, Robert R. Rich

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Abstract

Mta-specific cytotoxic T lymphocytes (CTL) can be generated in primary cultures of (NZB x B10.D2)F₁ spleen cells with H-2-compatible BALB/c stimulator cells. The CTL lyse reciprocal Mta⁺ (B10.D2 x NZB)F₁ as well as H-2-disparate targets, such as B10, B6, and B6-Tla^a; they do not lyse targets from NZB or any F₁ hybrid of an NZB mother. The lysis of ⁵¹Cr-labeled B10 targets is completely inhibited by unlabeled targets from Mta⁺ (B10.D2 x NZB)F₁, but not from the reciprocal Mta^-F₁, thus demonstrating H-2-unrestricted lysis of Mta.

Original language	English (US)
Pages (from-to)	1866-1871
Number of pages	6
Journal	Journal of Experimental Medicine
Volume	156
Issue number	6
DOIs	https://doi.org/10.1084/jem.156.6.1866
State	Published - Dec 1 1982

ASJC Scopus subject areas

General Medicine

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title = "Primary cell-mediated lympholysis response to a maternally transmitted antigen",

abstract = "Mta-specific cytotoxic T lymphocytes (CTL) can be generated in primary cultures of (NZB x B10.D2)F1 spleen cells with H-2-compatible BALB/c stimulator cells. The CTL lyse reciprocal Mta+ (B10.D2 x NZB)F1 as well as H-2-disparate targets, such as B10, B6, and B6-Tlaa; they do not lyse targets from NZB or any F1 hybrid of an NZB mother. The lysis of 51Cr-labeled B10 targets is completely inhibited by unlabeled targets from Mta+ (B10.D2 x NZB)F1, but not from the reciprocal Mta-F1, thus demonstrating H-2-unrestricted lysis of Mta.",

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doi = "10.1084/jem.156.6.1866",

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AU - Iii, Roger Smith

AU - Huston, David P.

AU - Rich, Robert R.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - Mta-specific cytotoxic T lymphocytes (CTL) can be generated in primary cultures of (NZB x B10.D2)F1 spleen cells with H-2-compatible BALB/c stimulator cells. The CTL lyse reciprocal Mta+ (B10.D2 x NZB)F1 as well as H-2-disparate targets, such as B10, B6, and B6-Tlaa; they do not lyse targets from NZB or any F1 hybrid of an NZB mother. The lysis of 51Cr-labeled B10 targets is completely inhibited by unlabeled targets from Mta+ (B10.D2 x NZB)F1, but not from the reciprocal Mta-F1, thus demonstrating H-2-unrestricted lysis of Mta.

AB - Mta-specific cytotoxic T lymphocytes (CTL) can be generated in primary cultures of (NZB x B10.D2)F1 spleen cells with H-2-compatible BALB/c stimulator cells. The CTL lyse reciprocal Mta+ (B10.D2 x NZB)F1 as well as H-2-disparate targets, such as B10, B6, and B6-Tlaa; they do not lyse targets from NZB or any F1 hybrid of an NZB mother. The lysis of 51Cr-labeled B10 targets is completely inhibited by unlabeled targets from Mta+ (B10.D2 x NZB)F1, but not from the reciprocal Mta-F1, thus demonstrating H-2-unrestricted lysis of Mta.

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Primary cell-mediated lympholysis response to a maternally transmitted antigen

Abstract

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