Mta-specific cytotoxic T lymphocytes (CTL) can be generated in primary cultures of (NZB x B10.D2)F1 spleen cells with H-2-compatible BALB/c stimulator cells. The CTL lyse reciprocal Mta+ (B10.D2 x NZB)F1 as well as H-2-disparate targets, such as B10, B6, and B6-Tlaa; they do not lyse targets from NZB or any F1 hybrid of an NZB mother. The lysis of 51Cr-labeled B10 targets is completely inhibited by unlabeled targets from Mta+ (B10.D2 x NZB)F1, but not from the reciprocal Mta-F1, thus demonstrating H-2-unrestricted lysis of Mta.
ASJC Scopus subject areas
- Immunology and Allergy