TY - JOUR
T1 - Pretreatment with tualang honey improves brain antioxidant/oxidant status of rats exposed to normobaric hypoxia
AU - Qaid, Entesar Yaseen Abdo
AU - Zakaria, Rahimah
AU - Yusof, Nurul Aiman Mohd
AU - Sulaiman, Shaida Fariza
AU - Romli, Aminah Che
AU - Shafin, Nazlahshaniza
AU - Ahmad, Asma Hayati
AU - Othman, Zahiruddin
AU - Muthuraju, Sangu
N1 - Funding Information:
We would like to acknowledge School of Medical Sciences, Universiti Sains Malaysia and short-term research grant of Universiti Sains Malaysia (304/PPSP/61313086).
Publisher Copyright:
© 2020 Japan Health Sciences University & Japan International Cultural Exchange Foundation.
PY - 2020
Y1 - 2020
N2 - Background: Tualang honey has been shown to reduce oxidative stress in various animal models. Objective: This study aims to investigate the responses of antioxidant defenses to normobaric hypoxia and the effects of Tualang honey on brain oxidant/antioxidant status of adult male rats. Methods: The rats were divided into four groups (n = 12 per group); i) sucrose non-hypoxia, ii) sucrose with hypoxia, iii) honey non-hypoxia, iv) honey with hypoxia. Oral Tualang honey (0.2 g/kg body weight) and sucrose (1 mL of 7.9%) supplementations were given to the rats daily for 14 days. The rats were then subjected to ~11% continuous normobaric hypoxia for 7 days. The brain homogenate was used to determine oxidative stress markers levels/activities. Results: The sucrose with hypoxia group demonstrated a significant increase in malondialdehyde (MDA) and a decrease in total antioxidant capacity (TAC), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels when compared to sucrose non-hypoxia group. A significant increase in TAC, CAT, SOD and GPx, and a decrease in MDA levels were observed especially in honey with hypoxia group compared to sucrose with hypoxia group. Conclusion: Our findings suggest that normobaric hypoxia disrupts the efficiency of the antioxidant system leading to oxidative damage. Pretreatment with Tualang honey has been demonstrated to increase brain tolerance to hypoxia.
AB - Background: Tualang honey has been shown to reduce oxidative stress in various animal models. Objective: This study aims to investigate the responses of antioxidant defenses to normobaric hypoxia and the effects of Tualang honey on brain oxidant/antioxidant status of adult male rats. Methods: The rats were divided into four groups (n = 12 per group); i) sucrose non-hypoxia, ii) sucrose with hypoxia, iii) honey non-hypoxia, iv) honey with hypoxia. Oral Tualang honey (0.2 g/kg body weight) and sucrose (1 mL of 7.9%) supplementations were given to the rats daily for 14 days. The rats were then subjected to ~11% continuous normobaric hypoxia for 7 days. The brain homogenate was used to determine oxidative stress markers levels/activities. Results: The sucrose with hypoxia group demonstrated a significant increase in malondialdehyde (MDA) and a decrease in total antioxidant capacity (TAC), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels when compared to sucrose non-hypoxia group. A significant increase in TAC, CAT, SOD and GPx, and a decrease in MDA levels were observed especially in honey with hypoxia group compared to sucrose with hypoxia group. Conclusion: Our findings suggest that normobaric hypoxia disrupts the efficiency of the antioxidant system leading to oxidative damage. Pretreatment with Tualang honey has been demonstrated to increase brain tolerance to hypoxia.
KW - Brain
KW - Hypoxia
KW - Oxidative stress
KW - Sucrose
KW - Tualang honey
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M3 - Article
AN - SCOPUS:85112147764
SN - 1341-2051
VL - 27
SP - 728
EP - 731
JO - International Medical Journal
JF - International Medical Journal
IS - 6
ER -