Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions

Philip S. Low; George N. Somero

doi:10.1016/0305-0491(75)90117-0

Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions

Philip S. Low, George N. Somero

Houston Methodist

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.

Original language	English (US)
Pages (from-to)	67-74
Number of pages	8
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/0305-0491(75)90117-0
State	Published - Sep 15 1975

Keywords

Abyssal fishes
Activation volumes
Hydrophobic interactions
Hydrostatic pressure
Pressure adaptation
Protein denaturation

ASJC Scopus subject areas

General Medicine

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10.1016/0305-0491(75)90117-0

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title = "Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions",

abstract = "1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.",

keywords = "Abyssal fishes, Activation volumes, Hydrophobic interactions, Hydrostatic pressure, Pressure adaptation, Protein denaturation",

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year = "1975",

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doi = "10.1016/0305-0491(75)90117-0",

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T1 - Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions

AU - Low, Philip S.

AU - Somero, George N.

PY - 1975/9/15

Y1 - 1975/9/15

N2 - 1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.

AB - 1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.

KW - Abyssal fishes

KW - Activation volumes

KW - Hydrophobic interactions

KW - Hydrostatic pressure

KW - Pressure adaptation

KW - Protein denaturation

UR - https://www.scopus.com/pages/publications/0016752928

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U2 - 10.1016/0305-0491(75)90117-0

DO - 10.1016/0305-0491(75)90117-0

M3 - Article

C2 - 1183178

AN - SCOPUS:0016752928

SN - 0305-0491

VL - 52

SP - 67

EP - 74

JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

IS - 1

ER -

Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions

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Keywords

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