TY - JOUR
T1 - Pressure effects on enzyme structure and function in vitro and under simulated in vivo conditions
AU - Low, Philip S.
AU - Somero, George N.
PY - 1975/9/15
Y1 - 1975/9/15
N2 - 1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.
AB - 1. 1. The sources and magnitudes of pressure effects on enzymic structure and function are discussed. 2. 2. Data suggest that, except for enzyme-ligand interactions, enzymes of abyssal organisms are highly similar to the homologous enzymes of shallow water and terrestrial species. 3. 3. The magnitude of pressure effects on enzymic processes is critically dependent on the experimental conditions used, including pH, ionic strength, ionic composition, ligand concentrations and temperature. The more closely in vitro conditions approach the intracellular milieu of the enzyme, the smaller is the effect of pressure on many enzymic properties.
KW - Abyssal fishes
KW - Activation volumes
KW - Hydrophobic interactions
KW - Hydrostatic pressure
KW - Pressure adaptation
KW - Protein denaturation
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U2 - 10.1016/0305-0491(75)90117-0
DO - 10.1016/0305-0491(75)90117-0
M3 - Article
C2 - 1183178
AN - SCOPUS:0016752928
VL - 52
SP - 67
EP - 74
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 0305-0491
IS - 1
ER -