Abstract
A "column-format" preparative electrophoresis device which obviates the need for gel extraction or secondary electro-elution steps is described. Separated biomolecules are continuously detected and eluted directly into a minimal volume of free solution for subsequent use. An optical fiber allows the species of interest to be detected just prior to elution from the gel column, and a small collection volume is created by addition of an ion-exchange membrane near the end of the column.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1739-1743 |
| Number of pages | 5 |
| Journal | Biotechnology Letters |
| Volume | 27 |
| Issue number | 22 |
| DOIs | |
| State | Published - Nov 2005 |
Keywords
- Membrane compartment
- Nafion
- On-column detection
- Optical fiber
- Preparative electrophoresis
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
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