Preparative electrophoresis with on-column optical fiber monitoring and direct elution into a minimized volume

George W. Jackson, Richard Willson

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A "column-format" preparative electrophoresis device which obviates the need for gel extraction or secondary electro-elution steps is described. Separated biomolecules are continuously detected and eluted directly into a minimal volume of free solution for subsequent use. An optical fiber allows the species of interest to be detected just prior to elution from the gel column, and a small collection volume is created by addition of an ion-exchange membrane near the end of the column.

Original languageEnglish (US)
Pages (from-to)1739-1743
Number of pages5
JournalBiotechnology Letters
Volume27
Issue number22
DOIs
StatePublished - Nov 2005

Keywords

  • Membrane compartment
  • Nafion
  • On-column detection
  • Optical fiber
  • Preparative electrophoresis

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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