Abstract
Logic-embedded vectors (LEVs) have been introduced as a means to overcome sequential, biological barriers that prevent particle-based drug delivery systems from reaching their targets. In this chapter, we address the challenge of fabricating and optimizing LEVs to reach non-endosomal targets. We describe the general preparation, characterization, and cellular association of porous silicon-based LEVs. A specific example of LEV fabrication from start to finish, along with optimization and troubleshooting information, is presented to serve as a template for future designs.
Original language | English |
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Pages (from-to) | 1-16 |
Number of pages | 16 |
Journal | Methods in Enzymology |
Volume | 508 |
DOIs | |
State | Published - Apr 2 2012 |
Keywords
- Chitosan
- Intracellular trafficking
- Iron oxide
- Microparticles
- Multistage
- Nanoparticles
- Porous silicon
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology