Preparation and properties of an apoprotein derivative of human serum β-lipoprotein

A. M. Gotto, R. I. Levy, D. S. Fredrickson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The aim of this study was to develop a convenient method for the preparation and study of a soluble delipidated form of human serum β-lipoprotein. This was achieved by succinylation and delipidation with ether-ethanol (3:1). The succinylated apoprotein was soluble in either 0.13 M Tris-HCl buffer, pH 8.2 (for β-lipoprotein prepared by ultracentrifugation) or in the same Tris buffer to which 5 mM sodium decyl sulfate was added (for heparin-Mn precipitated β-lipoprotein). The immunological activity of β-lipoprotein or its apoprotein were markedly altered by succinylation. Whereas the succinylated β-lipoprotein appeared as one peak in the analytical ultracentrifuge, the succinylated apoprotein appeared as two. Under the electron microscope β-lipoprotein and succinylated β-lipoprotein were indistinguishable, appearing as uniform preparations of spherical particles 215 to 220 A in diamater.

Original languageEnglish (US)
Pages (from-to)463-470
Number of pages8
JournalLipids
Volume3
Issue number6
DOIs
StatePublished - Nov 1968

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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