TY - JOUR
T1 - Preparation and characterization of PLGA loaded nanoparticles obtained from D. Melanoxylon Roxb. Leaves for their antiproliferative and antidiabetic activity
AU - Al Rashid, Md Harun
AU - Bharadwaj, P. V.P.Deepak
AU - Mandal, Vivekananda
AU - Pal, Mahadeb
AU - Mandal, Subhash C.
AU - Thandavarayan, Rajarajan Amirthalingam
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Introduction: Diospyros melanoxylon Roxb. is traditionally used medicinal plant. Previously, we confirmed the better antiproliferative and antidiabetic activity of ethyl acetate fractions (EAF) obtained from D. Melanoxylon. To assessment the hypothesis if nanoparticle-encapsulated fraction could progress bioactivity. Materials and Methods: We prepared nanoparticle encapsulation based on poly (lactide-co-glycolide) (PLGA) and confirmed encapsulation by scanning electron microscopy. Sub EAF (SEAF) and nanoparticles of sub ethyl acetate (NSEAF) were characterized for their antiproliferative and antidiabetic activity. Antiproliferative activity was performed using the methylene blue assay. Antidiabetic activity was performed on the basis of α-Amylase and α-glucosidase assay. Results and Discussion: Comparatively, NSEAF showed better activity on different cancerous cell lines HCT116 (inhibitory concentration 50% [IC50] 32.89 ± 1.71 μg/ml), MCF-7 (IC50 36.13 ± 0.96 μg/ml) and PC-3 (IC50 32.39 ± 1.91 μg/ml), and antidiabetic activity on the basis of α-Amylase (IC50 35.62 ± 2.56 μg/ml), and α- glucosidase (IC50 73.52 ± 1.13 μg/ml) action at a dose-dependent manner. It is also demonstrated that NSEAF exerted very low toxic effect against normal cell lines. Conclusion: For this reason, NSEAF studied in the present research may be considered as possible future drug candidates for the treatment and management of cancer and diabetes.
AB - Introduction: Diospyros melanoxylon Roxb. is traditionally used medicinal plant. Previously, we confirmed the better antiproliferative and antidiabetic activity of ethyl acetate fractions (EAF) obtained from D. Melanoxylon. To assessment the hypothesis if nanoparticle-encapsulated fraction could progress bioactivity. Materials and Methods: We prepared nanoparticle encapsulation based on poly (lactide-co-glycolide) (PLGA) and confirmed encapsulation by scanning electron microscopy. Sub EAF (SEAF) and nanoparticles of sub ethyl acetate (NSEAF) were characterized for their antiproliferative and antidiabetic activity. Antiproliferative activity was performed using the methylene blue assay. Antidiabetic activity was performed on the basis of α-Amylase and α-glucosidase assay. Results and Discussion: Comparatively, NSEAF showed better activity on different cancerous cell lines HCT116 (inhibitory concentration 50% [IC50] 32.89 ± 1.71 μg/ml), MCF-7 (IC50 36.13 ± 0.96 μg/ml) and PC-3 (IC50 32.39 ± 1.91 μg/ml), and antidiabetic activity on the basis of α-Amylase (IC50 35.62 ± 2.56 μg/ml), and α- glucosidase (IC50 73.52 ± 1.13 μg/ml) action at a dose-dependent manner. It is also demonstrated that NSEAF exerted very low toxic effect against normal cell lines. Conclusion: For this reason, NSEAF studied in the present research may be considered as possible future drug candidates for the treatment and management of cancer and diabetes.
KW - Antidiabetes
KW - Antiproliferative
KW - Diospyros melanoxylon
KW - Nanoparticles
KW - Poly (lactide-co-glycolide)
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M3 - Article
AN - SCOPUS:85034960459
VL - 11
SP - S438-S447
JO - International Journal of Green Pharmacy
JF - International Journal of Green Pharmacy
SN - 0973-8258
IS - 3
ER -