Prenatal administration of the cytochrome P4501A inducer, Β-naphthoflavone (BNF), attenuates hyperoxic lung injury in newborn mice: Implications for bronchopulmonary dysplasia (BPD) in premature infants

Xanthi I. Couroucli, Yan hong Wei Liang, Weiwu Jiang, Lihua Wang, Roberto Barrios, Peiying Yang, Bhagavatula Moorthy

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Supplemental oxygen contributes to the development of bronchopulmonary dysplasia (BPD) in premature infants. In this investigation, we tested the hypothesis that prenatal treatment of pregnant mice (C57BL/6J) with the cytochrome P450 (CYP)1A1 inducer, ß-napthoflavone (BNF), will lead to attenuation of lung injury in newborns (delivered from these dams) exposed to hyperoxia by mechanisms entailing transplacental induction of hepatic and pulmonary CYP1A enzymes. Pregnant mice were administered the vehicle corn oil (CO) or BNF (40mg/kg), i.p., once daily for 3days on gestational days (17-19), and newborns delivered from the mothers were either maintained in room air or exposed to hyperoxia (>95% O 2) for 1-5days. After 3-5days of hyperoxia, the lungs of CO-treated mice showed neutrophil infiltration, pulmonary edema, and perivascular inflammation. On the other hand, BNF-pretreated neonatal mice showed decreased susceptibility to hyperoxic lung injury. These mice displayed marked induction of ethoxyresorufin O-deethylase (EROD) (CYP1A1) and methoxyresorufin O-demethylase (MROD) (CYP1A2) activities, and levels of the corresponding apoproteins and mRNA levels until PND 3 in liver, while CYP1A1 expression alone was augmented in the lung. Prenatal BNF did not significantly alter gene expression of pulmonary NAD(P)H quinone reductase (NQO1). Hyperoxia for 24-72h resulted in increased pulmonary levels of the F 2-isoprostane 8-iso-PGF 2-, whose levels were decreased in mice prenatally exposed to BNF. In conclusion, our results suggest that prenatal BNF protects newborns against hyperoxic lung injury, presumably by detoxification of lipid hydroperoxides by CYP1A enzymes, a phenomenon that has implications for prevention of BPD in infants.

Original languageEnglish (US)
Pages (from-to)83-94
Number of pages12
JournalToxicology and Applied Pharmacology
Volume256
Issue number2
DOIs
StatePublished - Oct 15 2011

Keywords

  • Bronchopulmonary dysplasia
  • CYP1A enzymes
  • Hyperoxia
  • Lung injury
  • Newborn
  • Oxidant injury

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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