Preferential glial cell attachment to microcontact printed surfaces

Pamela M. St. John; Lance Kam; Stephen W. Turner; Harold G. Craighead; Michael Issacson; James N. Turner; William Shain

doi:10.1016/S0165-0270(97)00069-1

Preferential glial cell attachment to microcontact printed surfaces

Pamela M. St. John, Lance Kam, Stephen W. Turner, Harold G. Craighead, Michael Issacson, James N. Turner, William Shain

Academic Institute

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Microcontact printing is introduced as a method for fabricating test surfaces for attachment of cells to chemically patterned silicon surfaces. Tests with astroglial cells indicate that cells attach to microcontact printed surfaces similarly to surfaces produced by traditional photolithographic methods. Astroglial cells attach selectively to 50 μm wide bars of N¹[3(Trimethoxysi-lyl)propyl]diethylenetriamine (DETA) self- assembled monolayers (SAMs) on surfaces prepared using variable width spaces generated from microcontact printing with octadecyltrichlorosilane (OTS) as the ink. Our results demonstrate that microcontact printing provides an effective and rapid method for routine production of patterned self-assembled monolayers that can be used for directing cell attachment and studying cell morphology.

Original language	English (US)
Pages (from-to)	171-177
Number of pages	7
Journal	Journal of Neuroscience Methods
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/S0165-0270(97)00069-1
State	Published - Aug 22 1997

Keywords

Astroglia
Cell attachment
Cell culture
Microcontact printing
Self-assembled monolayer

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0165-0270(97)00069-1

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title = "Preferential glial cell attachment to microcontact printed surfaces",

abstract = "Microcontact printing is introduced as a method for fabricating test surfaces for attachment of cells to chemically patterned silicon surfaces. Tests with astroglial cells indicate that cells attach to microcontact printed surfaces similarly to surfaces produced by traditional photolithographic methods. Astroglial cells attach selectively to 50 μm wide bars of N1[3(Trimethoxysi-lyl)propyl]diethylenetriamine (DETA) self- assembled monolayers (SAMs) on surfaces prepared using variable width spaces generated from microcontact printing with octadecyltrichlorosilane (OTS) as the ink. Our results demonstrate that microcontact printing provides an effective and rapid method for routine production of patterned self-assembled monolayers that can be used for directing cell attachment and studying cell morphology.",

keywords = "Astroglia, Cell attachment, Cell culture, Microcontact printing, Self-assembled monolayer",

author = "{St. John}, {Pamela M.} and Lance Kam and Turner, {Stephen W.} and Craighead, {Harold G.} and Michael Issacson and Turner, {James N.} and William Shain",

note = "Funding Information: This work was supported in part by National Institutes of Health's National Center for Research Resources grant R01RR1095701. The authors would like to acknowledge the use of the facilities at the Cornell Nanofabrication Facility and the Cornell Materials Science Center and the contributions of D. Szarowski.",

year = "1997",

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doi = "10.1016/S0165-0270(97)00069-1",

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T1 - Preferential glial cell attachment to microcontact printed surfaces

AU - St. John, Pamela M.

AU - Kam, Lance

AU - Turner, Stephen W.

AU - Craighead, Harold G.

AU - Issacson, Michael

AU - Turner, James N.

AU - Shain, William

N1 - Funding Information: This work was supported in part by National Institutes of Health's National Center for Research Resources grant R01RR1095701. The authors would like to acknowledge the use of the facilities at the Cornell Nanofabrication Facility and the Cornell Materials Science Center and the contributions of D. Szarowski.

PY - 1997/8/22

Y1 - 1997/8/22

N2 - Microcontact printing is introduced as a method for fabricating test surfaces for attachment of cells to chemically patterned silicon surfaces. Tests with astroglial cells indicate that cells attach to microcontact printed surfaces similarly to surfaces produced by traditional photolithographic methods. Astroglial cells attach selectively to 50 μm wide bars of N1[3(Trimethoxysi-lyl)propyl]diethylenetriamine (DETA) self- assembled monolayers (SAMs) on surfaces prepared using variable width spaces generated from microcontact printing with octadecyltrichlorosilane (OTS) as the ink. Our results demonstrate that microcontact printing provides an effective and rapid method for routine production of patterned self-assembled monolayers that can be used for directing cell attachment and studying cell morphology.

AB - Microcontact printing is introduced as a method for fabricating test surfaces for attachment of cells to chemically patterned silicon surfaces. Tests with astroglial cells indicate that cells attach to microcontact printed surfaces similarly to surfaces produced by traditional photolithographic methods. Astroglial cells attach selectively to 50 μm wide bars of N1[3(Trimethoxysi-lyl)propyl]diethylenetriamine (DETA) self- assembled monolayers (SAMs) on surfaces prepared using variable width spaces generated from microcontact printing with octadecyltrichlorosilane (OTS) as the ink. Our results demonstrate that microcontact printing provides an effective and rapid method for routine production of patterned self-assembled monolayers that can be used for directing cell attachment and studying cell morphology.

KW - Astroglia

KW - Cell attachment

KW - Cell culture

KW - Microcontact printing

KW - Self-assembled monolayer

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Preferential glial cell attachment to microcontact printed surfaces

Abstract

Keywords

ASJC Scopus subject areas

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