An improved magnetic repulsion-actuated method is presented for precise control of trapping and release of individual pi-coliter droplets in comb-shaped microfluidic chip. The initial distance between droplets and microwells are same and maximized by forcing droplets to flow along a straight microchannel wall, making single droplet easier to be selected and trapped into designated microwell. Trapped droplets are isolated from ferrofluid by utilizing the effect of laminar flow between ferrofluid and mineral oil, effectively reducing undesired interference from magnetic repulsion to adjacent droplets. Moreover, ferrofluid can be completely displaced by mineral oil for eliminating adverse effects from surfactants.
|Original language||English (US)|
|Title of host publication||15th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|State||Published - 2011|