Potentiometric monitoring DNA hybridization

Y. Zhou, B. Yu, Anthony Guiseppi-Elie, V. Sergeyev, K. Levon

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The usual procedure to monitor the ion exchange of small ions utilizes a potentiometer with a selective membrane as part of the working electrode. As the next step, we have applied polyaniline electrodes to the monitoring the activity macromolecular ions during DNA hybridization. Single-strand oligonucleotide (ssODN) probes were immobilized using a nucleophilic substitution reaction of the thiolated ssODN molecules with polyaniline. The anionic phosphate groups of the probe molecules also interacted with the cationic-doped polyaniline surface. Three useful findings were observed with the potentiometric experiments. First, the binding of the complimentary target molecules with the immobilized probes revealed a substantial potential change. Further, potential change was observed neither with the non-complimentary targets nor with the samples with a mutation in the sequence. The last two experiments were important for the future evaluation of the impact of medium and potential interfering compounds: anionic groups and hydrogen bonding groups in the non-complimentary samples did not cause any interactions.

Original languageEnglish (US)
Pages (from-to)3275-3280
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number11
DOIs
StatePublished - Jul 15 2009

Keywords

  • DNA hybridization
  • Polyaniline
  • Potentiometry

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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