Potential role of activated platelets in homing of human endothelial progenitor cells to subendothelial matrix

Eli I. Lev, Zeev Estrov, Khatira Aboulfatova, David Harris, Juan F. Granada, Carlos Alviar, Neal Kleiman, Jing Fei Dong

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


Endothelial progenitor cells (EPCs) mobilize from the bone marrow in response to tissue injury and participate in vascular repair. However, there is limited data about the homing mechanisms of EPCs to vascular injury sites. Recently animal experiments indicated that platelets play a role in recruitment of EPCs to injury sites. However, data on the possible interaction between platelets and EPCs within the human system are limited. We, therefore, examined in-vitro human platelet-EPC interaction under static and flow conditions. Human EPCs were isolated from donated buffy coats by magnetic microbeads and flow cytometry cell sorting using CDI33 and VEGFR-2, respectively, as markers. Platelets were tested in the form of washed platelets, platelet rich plasma or whole blood. EPCs formed heterotypic aggregates with resting platelets under static conditions, an interaction that was greatly enhanced when platelets were activated by collagen,AIDP or thrombin-activation peptide.The platelet-EPC interaction was inhibited by antibodies to P-selectin or P-selectin glycoprotein ligand-I (PSGL-I), but not by antibodies to glycoproteins Ib-IX-V or IIb/IIIa. When perfused over activated platelets under shear stress of 2.5 dyn/cm2, EPCs tethered to platelayers and either adhered immediately or rolled a short distance before adhering. In addition, platelets promoted the colonization of adherent EPCs in culture conditions. Consistent with recent animal studies, these findings demonstrate that human EPCs interact in vitro with activated platelets under static and flow conditions, mediated through P-selectin-PSGL-I interaction. This interaction may be a central mechanism for homing of EPCs to vascular injury sites.

Original languageEnglish (US)
Pages (from-to)498-504
Number of pages7
JournalThrombosis and Haemostasis
Issue number4
StatePublished - Oct 1 2006


  • Endothelial progenitor cells
  • Interaction
  • Platelets

ASJC Scopus subject areas

  • Hematology


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