Portable FRET sensing of proteins, hormones, and toxins using DNA aptamers and quantum dots

G. W. Jackson, U. Strych, E. Frank, Richard C. Willson, R. Ballerstadt, R. J. McNichols

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

There is an urgent need to develop methods and devices that make the monitoring of any compound of interest in the public water system more reliable, more sensitive and more economical. We are developing a platform sensing chemistry based on rapidly responsive fluorescent resonance energy transfer, or FRET with DNA aptamers as molecular recognition elements. In particular, we are using highly stable, highly fluorescent quantum dots and have implemented the sensing chemistry in a format compatible with readout by an inexpensive, portable, and yet highly sensitive fluorometer. Using different aptamers in the sensor formulation, we demonstrate the sensing and quantitation of the protein insulin and the small molecule hormone, thyroxine (T4). We are presently formulating similar sensors for the potent cyanobacterial toxin, anatoxin-a. Building on extensive previous glucose sensing experience, we also look towards the necessary modifications to develop a fully reversible, on-line sensor for similar analytes.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages205-208
Number of pages4
Volume2
StatePublished - Dec 1 2009
EventNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
Duration: May 3 2009May 7 2009

Other

OtherNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
CountryUnited States
CityHouston, TX
Period5/3/095/7/09

Keywords

  • Aptamers
  • FRET
  • Portable sensing
  • Quantum dots

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials

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