Poor Sensitivity of the MALDI Biotyper® MBT Subtyping Module for Detection of Klebsiella pneumoniae Carbapenemase (KPC) in Klebsiella Species

Luz Cuello; Judith Alvarez Otero; Kerryl E. Greenwood-Quaintance; Liang Chen; Blake Hanson; Jinnethe Reyes; Lauren Komarow; Lizhao Ge; Zane D. Lancaster; Garrett G. Gordy; Audrey N. Schuetz; Robin Patel

doi:10.3390/antibiotics12091465

Poor Sensitivity of the MALDI Biotyper^® MBT Subtyping Module for Detection of Klebsiella pneumoniae Carbapenemase (KPC) in Klebsiella Species

Luz Cuello, Judith Alvarez Otero, Kerryl E. Greenwood-Quaintance, Liang Chen, Blake Hanson, Jinnethe Reyes, Lauren Komarow, Lizhao Ge, Zane D. Lancaster, Garrett G. Gordy, Audrey N. Schuetz, Robin Patel

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Rapid detection of Klebsiella pneumoniae carbapenemase (KPC) in the Klebsiella species is desirable. The MALDI Biotyper^® MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the p019 gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical Klebsiella isolates, with whole genome sequences used to assess for bla_KPC and p019. For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring p019, the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of bla_KPC-positive Klebsiella isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of p019 being present in only 43% of the bla_KPC-positive Klebsiella isolates.

Original language	English (US)
Article number	1465
Journal	Antibiotics
Volume	12
Issue number	9
DOIs	https://doi.org/10.3390/antibiotics12091465
State	Published - Sep 2023

Keywords

carbapenem resistance
CRE
Klebsiella
KPC
MALDI-ToF mass spectrometry

ASJC Scopus subject areas

Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

Access to Document

10.3390/antibiotics12091465

Cite this

Cuello, L., Alvarez Otero, J., Greenwood-Quaintance, K. E., Chen, L., Hanson, B., Reyes, J., Komarow, L., Ge, L., Lancaster, Z. D., Gordy, G. G., Schuetz, A. N., & Patel, R. (2023). Poor Sensitivity of the MALDI Biotyper^® MBT Subtyping Module for Detection of Klebsiella pneumoniae Carbapenemase (KPC) in Klebsiella Species. Antibiotics, 12(9), Article 1465. https://doi.org/10.3390/antibiotics12091465

Cuello, L, Alvarez Otero, J, Greenwood-Quaintance, KE, Chen, L, Hanson, B, Reyes, J, Komarow, L, Ge, L, Lancaster, ZD, Gordy, GG, Schuetz, AN & Patel, R 2023, 'Poor Sensitivity of the MALDI Biotyper^® MBT Subtyping Module for Detection of Klebsiella pneumoniae Carbapenemase (KPC) in Klebsiella Species', Antibiotics, vol. 12, no. 9, 1465. https://doi.org/10.3390/antibiotics12091465

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abstract = "Rapid detection of Klebsiella pneumoniae carbapenemase (KPC) in the Klebsiella species is desirable. The MALDI Biotyper{\textregistered} MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the p019 gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical Klebsiella isolates, with whole genome sequences used to assess for blaKPC and p019. For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring p019, the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of blaKPC-positive Klebsiella isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of p019 being present in only 43% of the blaKPC-positive Klebsiella isolates.",

keywords = "carbapenem resistance, CRE, Klebsiella, KPC, MALDI-ToF mass spectrometry",

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AU - Greenwood-Quaintance, Kerryl E.

AU - Chen, Liang

AU - Hanson, Blake

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AU - Komarow, Lauren

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AU - Schuetz, Audrey N.

AU - Patel, Robin

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