Polypyrrole-hydrogel composites for the construction of clinically important biosensors

Sean Brahim, Dyer Narinesingh, Anthony Guiseppi-Elie

Research output: Contribution to journalArticle

151 Scopus citations

Abstract

The present study reports on the use of p(2-hydroxyethyl methacrylate) (pHEMA) in which polypyrrole and various oxidoreductase enzymes were physically entrapped to function as a viable matrix for the construction of clinically important amperometric biosensors. Glucose oxidase, cholesterol oxidase and galactose oxidase biosensors were constructed. Electrode-supported hydrogel films were prepared by UV polymerization of the HEMA component (containing the dissolved enzyme) followed immediately by electrochemical polymerization (+0.7V vs. Ag/AgCl) of the pyrrole component within the interstitial spaces of the pre-formed hydrogel network. The optimized glucose oxidase biosensor displayed a wide linear glucose response range (5.0 × 10-5 to 2.0 × 10-2 M), a detection limit (3Sy/x/sensitivity) of 25 μM and a response time of 35-40 s. The analytical recovery of glucose in serum samples ranged from 98 to 102% with mean coefficients of variation of 4.4% (within-day analyses) and 5.1% (day-to-day analyses). All three sensors displayed good stabilities when stored desiccated in the absence of buffer (> 9 months).

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalBiosensors and Bioelectronics
Volume17
Issue number1-2
DOIs
StatePublished - 2002

Keywords

  • Amperometric biosensor
  • Cholesterol oxidase
  • Galactose oxidase
  • Glucose oxidase
  • Polypyrrole
  • p(HEMA)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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