Abstract
The present study reports on the use of p(2-hydroxyethyl methacrylate) (pHEMA) in which polypyrrole and various oxidoreductase enzymes were physically entrapped to function as a viable matrix for the construction of clinically important amperometric biosensors. Glucose oxidase, cholesterol oxidase and galactose oxidase biosensors were constructed. Electrode-supported hydrogel films were prepared by UV polymerization of the HEMA component (containing the dissolved enzyme) followed immediately by electrochemical polymerization (+0.7V vs. Ag/AgCl) of the pyrrole component within the interstitial spaces of the pre-formed hydrogel network. The optimized glucose oxidase biosensor displayed a wide linear glucose response range (5.0 × 10-5 to 2.0 × 10-2 M), a detection limit (3Sy/x/sensitivity) of 25 μM and a response time of 35-40 s. The analytical recovery of glucose in serum samples ranged from 98 to 102% with mean coefficients of variation of 4.4% (within-day analyses) and 5.1% (day-to-day analyses). All three sensors displayed good stabilities when stored desiccated in the absence of buffer (> 9 months).
Original language | English (US) |
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Pages (from-to) | 53-59 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 17 |
Issue number | 1-2 |
DOIs | |
State | Published - 2002 |
Keywords
- Amperometric biosensor
- Cholesterol oxidase
- Galactose oxidase
- Glucose oxidase
- Polypyrrole
- p(HEMA)
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry