Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells

Barbara Adinolfi, Francesco Baldini, Ambra Giannetti, Sara Tombelli, Cosimo Trono, Giovanna Sotgiu, Greta Varchi, Mario Pellegrino, Claudio Domenici

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of antisense oligonucleotide molecular beacons, able to generate a fluorescent signal when they hybridize with their target mRNA, represents an innovative strategy in cancer. This approach is able to conjugate the ability of sensing specific mRNA with the pharmacological silencing activity, preventing the overexpression of proteins associated to cancer development. In this context, this strategy minimizes the non-specific toxicity addressing the therapy mainly towards the tumor cells by using effective delivery systems. The aim of this work was to investigate the ability of polymethylmethacrylate nanoparticles (PMMA-NPs) to act as vehicle of an oligonucleotide molecular beacon (MB) targeting survivin mRNA in A549 human lung adenocarcinoma epithelial cells. Furthermore, this paper focuses the attention on the need for having an appropriate healthy control in in-vitro experiments. In particular, the survivin-MB was firstly characterized in solution in order to verify its functionality and then the PMMA-NPs ability to promote the MB internalization was verified in A549 cells by confocal microscopy. Confluent Human Dermal Fibroblasts from adult (HDFa) were used as healthy control. The results showed that PMMA-NPs promote the survivin-MB cellular up-take and that the use of 10 μg/mL PMMA-NPs as carrier for survivin-MB for 1h 30 min might be a promising strategy to reduce cancer cell proliferation avoiding detectable consequences on the healthy cells.

Original languageEnglish (US)
Title of host publication2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages35-38
Number of pages4
ISBN (Print)9781509051519
DOIs
StatePublished - Aug 3 2018
Event1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015 - Lecce, Italy
Duration: Jul 24 2015Jul 25 2015

Publication series

Name2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015

Other

Other1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015
CountryItaly
CityLecce
Period7/24/157/25/15

Keywords

  • cancer cells
  • fibroblasts
  • molecular beacon
  • nanoparticles
  • survivin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation

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