Polymer nanoparticles with electrostatically loaded multicargo for combined cancer phototherapy

Aurore Fraix, Ilse Manet, Marco Ballestri, Andrea Guerrini, Paolo Dambruoso, Giovanna Sotgiu, Greta Varchi, Monica Camerin, Olimpia Coppellotti, Salvatore Sortino

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We have developed a multifunctional polymer-based nanoplatform for bimodal cancer phototherapy. It was achieved by electrostatic entangling of two anionic photoactivable components, a commercial porphyrin and a tailored nitro-aniline derivative, within the cationic shell of polymeric nanoparticles (NPs) based on polymethyl methacrylate. The combination of steady-state and time-resolved spectroscopic and photochemical techniques shows that the two photoresponsive agents do not interfere with each other while being in close proximity in the same polymeric scaffold and can thus operate in parallel under the exclusive control of light stimuli. Specifically, visible light triggers satisfactory red fluorescence emission and generation of singlet oxygen (1O2) from one component and release of nitric oxide (NO) from the other. Fluorescence microscopy analysis provides unambiguous evidence for the internalization of the NPs within B78H1 melanoma cells, where they induce amplified mortality due to a combinatory effect of the two photogenerated cytotoxic species. This journal is

Original languageEnglish (US)
Pages (from-to)3001-3010
Number of pages10
JournalJournal of Materials Chemistry B
Volume3
Issue number15
DOIs
StatePublished - Apr 21 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

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