Platinum-Decorated Gold Nanoparticle-Based Microfluidic Chip Immunoassay for Ultrasensitive Colorimetric Detection of SARS-CoV-2 Nucleocapsid Protein

Feng Wu, Mao Mao, Liangyu Cai, Qianyu Lin, Xuejiao Guan, Xueying Shi, Lan Ma

Research output: Contribution to journalArticlepeer-review

Abstract

Gold nanoparticle-based point-of-care tests (POCT) are one of the most widely used diagnostic tools for SARS-CoV-2 screening. However, the limitation of their insufficient sensitivity often leads to false negative results in early disease diagnostics. The ongoing pandemic of COVID-19 makes diagnostic tools that are more accurate, sensitive, simple, and affordable in high demand. In this work, we develop a platinum-decorated gold nanoparticle (Au@Pt NP)-based microfluidic chip immunoassay with a sensitivity surpassing that of paper-based detection of nucleocapsid (N) protein, one of the most conserved biomarkers of COVID-19. The synthesized Au@Pt NPs show high stability and catalytic activity in complex environments. The catalytic amplification of Au@Pt NPs enables naked-eye detection of N protein in the low femtogram range (ca. 0.1 pg/mL) and the detection of throat swab samples in under 40 min. This microfluidic chip immunoassay is easy for operation and readout without instrument assistance, making it more suitable for on-site detection and future pathogen surveillance.

Original languageEnglish (US)
Pages (from-to)3924-3932
Number of pages9
JournalACS Biomaterials Science and Engineering
Volume8
Issue number9
DOIs
StatePublished - Sep 12 2022

Keywords

  • COVID-19
  • immunoassay
  • microfluidic
  • nanoparticle
  • nucleocapsid protein

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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