Platforms to test the temporospatial capabilities of carrier systems in delivering growth factors to benefit vascular bioengineering

Lissenya B. Argueta, Jean A. Niles, Jason Sakamoto, Xuewu Liu, Stephanie P. Vega, Luba Frank, Marco Paessler, Joaquin Cortiella, Joan E. Nichols

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we produced a set of in vitro culture platforms to model vascular cell responses to growth factors and factor delivery vehicles. Two of the systems (whole vessel and whole lung vascular development) were supported by microfluidic systems facilitating media circulation and waste removal. We assessed vascular endothelial growth factor (VEGF) delivery by Pluronic F-127 hydrogel, 30 nm pore-sized microparticles (MPs), 60 nm pore-sized MP or a 50/50 mixture of 30 and 60 nm pore-sized MP. VEGF was delivered to porcine acellular lung vascular scaffolds (2.5 cm2 square pieces or whole 3D segments of acellular blood vessels) as well as whole acellular lung scaffolds. Scaffold-cell attachment was examined as was vascular tissue formation. We showed that a 50/50 mixture of 30 and 60 nm pore-sized silicon wafer MPs allowed for long-term release of VEGF within the scaffold vasculature and supported vascular endothelial tissue development during in vitro culture.

Original languageEnglish (US)
Article number102419
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume36
DOIs
StatePublished - Aug 2021

Keywords

  • Acellular scaffolds
  • Bioengineered lung
  • Bioreactor culture
  • Microfluidic platforms
  • Microparticle delivery
  • VEGF

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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