Abstract
Cell and gene cancer therapies require ex vivo cell processing of human grafts. Such processing requires at least three steps - cell enrichment, cell separation (destruction), and gene transfer - each of which requires the use of a separate technology. While these technologies may be satisfactory for research use, they are of limited usefulness in the clinical treatment setting because they have a low processing rate, as well as a low transfection and separation efficacy and specificity in heterogeneous human grafts. Most problematic, because current technologies are administered in multiple steps - rather than in a single, multifunctional, and simultaneous procedure - they lengthen treatment process and introduce an unnecessary level of complexity, labor, and resources into clinical treatment; all these limitations result in high losses of valuable cells. We report a universal, high-throughput, and multifunctional technology that simultaneously (1) inject free external cargo in target cells, (2) destroys unwanted cells, and (3) preserve valuable non-target cells in heterogeneous grafts. Each of these functions has single target cell specificity in heterogeneous cell system, processing rate > 45 mln cell/min, injection efficacy 90% under 96% viability of the injected cells, target cell destruction efficacy > 99%, viability of not-target cells >99% The developed technology employs novel cellular agents, called plasmonic nanobubbles (PNBs). PNBs are not particles, but transient, intracellular events, a vapor nanobubbles that expand and collapse in mere nanoseconds under optical excitation of gold nanoparticles with short picosecond laser pulses. PNBs of different, cell-specific, size (1) inject free external cargo with small PNBs, (2) Destroy other target cells mechanically with large PNBs and (3) Preserve non-target cells. The multi-functionality, precision, and high throughput of all-in-one PNB technology will tremendously impact cell and gene therapies and other clinical applications that depend on ex vivo processing of heterogeneous cell systems.
Original language | English (US) |
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Title of host publication | Frontiers in Ultrafast Optics |
Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XIV |
Publisher | SPIE |
Volume | 8972 |
ISBN (Print) | 9780819498854 |
DOIs | |
State | Published - Jan 1 2014 |
Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - San Francisco, CA, United States Duration: Feb 2 2014 → Feb 5 2014 |
Other
Other | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV |
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Country/Territory | United States |
City | San Francisco, CA |
Period | 2/2/14 → 2/5/14 |
Keywords
- Cell separation
- Cell therapy
- Delivery
- Gold nanoparticle
- Laser
- Plasmonic nanobubble
ASJC Scopus subject areas
- Applied Mathematics
- Computer Science Applications
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics