Plasma catabolism of human apolipoprotein E isoproteins: Lack of conversion of the doubly sialilated form to the asialo form in plasma

Giancarlo Ghiselli, Yitzhak Beigel, Maurizio Soma, Antonio Gotto

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Apolipoprotein E (apoE) circulates as a mixture of sialilated and asialilated forms. In this study the catabolic fate and the plasma turnover rate of the different apoE forms have been investigated in vivo in humans. Asialo apoE (E) and doubly sialiated apoE (Ess) were isolated by preparative isoelectrofocusing from the VLDL of subjects homozygous for the E3 allele. 131E3 and 125E3ss were injected simultaneously into three hypertriglyceridemic subjects, and plasma samples were collected up to the sixth day. VLDL were isolated by ultracentrifugation, and the apoE forms were separated by isoelectrofocusing. Gel bands corresponding to E3 and E3ss were cut out and counted for the associated radioactivity. Residence times in plasma for 131E3 and 125E3ss were 0.95 ± 0.16 and 0.74 ± 0.16 days, respectively. As determined from the gel count distribution up to 24 hours, no conversion of the injected sialilated form to the correspondent asialilated form was detected.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalMetabolism
Volume35
Issue number5
DOIs
StatePublished - May 1986

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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