TY - JOUR
T1 - PI3K/AKT/mTOR hypersignaling in autoimmune lymphoproliferative disease engendered by the epistatic interplay of Sle1b and FASlpr
AU - Xie, Chun
AU - Patel, Rahul
AU - Wu, Tianfu
AU - Zhu, Jiankun
AU - Henry, Tamika
AU - Bhaskarabhatla, Madhavi
AU - Samudrala, Renuka
AU - Tus, Katalin
AU - Gong, Yimei
AU - Zhou, Hui
AU - Wakeland, Edward K.
AU - Zhou, Xin J.
AU - Mohan, Chandra
N1 - Funding Information:
This work is supported in part by the National Institutes of Health grants RO1 AR44894, the Alliance for Lupus Research, as well as the National Arthritis Foundation.
PY - 2007/4
Y1 - 2007/4
N2 - Previous studies have demonstrated that the NZM2410/NZW 'z' allele of Sle1 on telomeric murine chromosome 1 led to lymphoproliferative autoimmunity, when acting in concert with the FASlpr defect on the C57BL/6 background. The present report shows that the Sle1b sub-locus, harboring the NZM2410/NZW 'z' allele of SLAM, in epistasis with FASlpr, may be sufficient to induce lymphoproliferative autoimmunity. Disease in this simplified genetic model is accompanied by significant activation of the AKT signaling axis in both B- and T cells, as evidenced by increased phosphorylation of AKT, mTOR, 4EBP-1 and p70S6K, resulting from increased PI3K and reduced PTEN activity. In addition, blocking this axis using RAD001, an mTOR inhibitor, ameliorated lymphoproliferation and modulated serum IgG anti-nuclear auto-antibodies. Finally, mTOR inhibition also dampened signaling via parallel axes, including the MAPK and NFkB pathways. Hence, hypersignaling via the PI3K/AKT/mTOR axis appears to be an important mechanism underlying autoimmune lymphoproliferative disease, presenting itself as a potential target for therapeutic intervention.
AB - Previous studies have demonstrated that the NZM2410/NZW 'z' allele of Sle1 on telomeric murine chromosome 1 led to lymphoproliferative autoimmunity, when acting in concert with the FASlpr defect on the C57BL/6 background. The present report shows that the Sle1b sub-locus, harboring the NZM2410/NZW 'z' allele of SLAM, in epistasis with FASlpr, may be sufficient to induce lymphoproliferative autoimmunity. Disease in this simplified genetic model is accompanied by significant activation of the AKT signaling axis in both B- and T cells, as evidenced by increased phosphorylation of AKT, mTOR, 4EBP-1 and p70S6K, resulting from increased PI3K and reduced PTEN activity. In addition, blocking this axis using RAD001, an mTOR inhibitor, ameliorated lymphoproliferation and modulated serum IgG anti-nuclear auto-antibodies. Finally, mTOR inhibition also dampened signaling via parallel axes, including the MAPK and NFkB pathways. Hence, hypersignaling via the PI3K/AKT/mTOR axis appears to be an important mechanism underlying autoimmune lymphoproliferative disease, presenting itself as a potential target for therapeutic intervention.
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U2 - 10.1093/intimm/dxm017
DO - 10.1093/intimm/dxm017
M3 - Article
C2 - 17369192
AN - SCOPUS:34047170343
SN - 0953-8178
VL - 19
SP - 509
EP - 522
JO - International Immunology
JF - International Immunology
IS - 4
ER -