A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an Mr of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Chemistry
- Health, Toxicology and Mutagenesis