Physicochemical and immunochemical characterization of a binding-protein for PCB methyl sulfones

Johan Lund, Lena Nordlund, Theodora Devereux, Hans Glaumann, Jan-Ake Gustafsson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an Mr of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.

Original languageEnglish (US)
Pages (from-to)1677-1680
Number of pages4
JournalChemosphere
Volume16
Issue number8-9
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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