Physicochemical and immunochemical characterization of a binding-protein for PCB methyl sulfones

Johan Lund; Lena Nordlund; Theodora Devereux; Hans Glaumann; Jan-Ake Gustafsson

doi:10.1016/0045-6535(87)90148-2

Physicochemical and immunochemical characterization of a binding-protein for PCB methyl sulfones

Johan Lund, Lena Nordlund, Theodora Devereux, Hans Glaumann, Jan-Ake Gustafsson

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Abstract

A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an M_r of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.

Original language	English (US)
Pages (from-to)	1677-1680
Number of pages	4
Journal	Chemosphere
Volume	16
Issue number	8-9
DOIs	https://doi.org/10.1016/0045-6535(87)90148-2
State	Published - Jan 1 1987

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
Chemistry(all)
Pollution
Health, Toxicology and Mutagenesis

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10.1016/0045-6535(87)90148-2

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abstract = "A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an Mr of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.",

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T1 - Physicochemical and immunochemical characterization of a binding-protein for PCB methyl sulfones

AU - Lund, Johan

AU - Nordlund, Lena

AU - Devereux, Theodora

AU - Glaumann, Hans

AU - Gustafsson, Jan-Ake

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an Mr of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.

AB - A binding protein for certain methylsulfonyl-metabolites of polychlorinated biphenyls (methylsulfonyl-PCBs) was purified from rat lung cytosol. The binding protein was found to have an Mr of 13,000 (13K) and to consist of subunits held together by disulfide bonds. Specific antibodies to the 13K-protein were raised in rabbits. The antibodies were used for immunohistochemical analysis of rat lung sections and Western immunoblot analysis of cytosolic preparations from enriched populations of rat lung cells. These experiments demonstrated that the 13K methylsulfonyl-PCB binding protein is localized primarily in the Clara cells of the rat airway epithelium and in the material lining the airways.

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Physicochemical and immunochemical characterization of a binding-protein for PCB methyl sulfones

Abstract

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