TY - JOUR
T1 - Photoluminescent Molecules and Materials as Diagnostic Reporters in Lateral Flow Assays
AU - Danthanarayana, Adheesha N.
AU - Brgoch, Jakoah
AU - Willson, Richard C.
N1 - Funding Information:
This work was funded in part by NIAID/NIH (grant nos. 1R43AI118180-01A1 and 1R01AR072742-01) and CDC (grant no. 1U01CK000512-01 and contract no. 200-2017-M-94591). The authors also acknowledge the NIH Rapid Acceleration of Diagnostics (RADx) Project no. 7720 and DOD CDMRP W81XWH-21-1-0975 (CA200041). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH, NIAID, or CDC.
Publisher Copyright:
©
PY - 2022/1/17
Y1 - 2022/1/17
N2 - The lateral flow assay (LFA) is a point-of-care diagnostic test commonly available in an over-the-counter format because of its simplicity, speed, low cost, and portability. The reporter particles in these assays are among their most significant components because they perform the diagnostic readout and dictate the test's sensitivity. Today, gold nanoparticles are frequently used as reporters, but recent work focusing on photoluminescent-based reporter technologies has pushed LFAs to better performance. These efforts have focused specifically on reporters made of organic fluorophores, quantum dots, lanthanide chelates, persistent luminescent phosphors, and upconversion phosphors. In most cases, photoluminescent reporters show enhanced sensitivity compared to conventional gold nanoparticle-based assays. Here, we examine the advantages and disadvantages of these different reporters and highlight their potential benefits in LFAs. Our assessment shows that photoluminescent-based LFAs can not only reach lower detection limits than LFAs with traditional reporters, but they also can be capable of quantitative and multiplex analyte detection. As a result, the photoluminescent reporters make LFAs well-suited for medical diagnostics, the food and agricultural industry, and environmental testing.
AB - The lateral flow assay (LFA) is a point-of-care diagnostic test commonly available in an over-the-counter format because of its simplicity, speed, low cost, and portability. The reporter particles in these assays are among their most significant components because they perform the diagnostic readout and dictate the test's sensitivity. Today, gold nanoparticles are frequently used as reporters, but recent work focusing on photoluminescent-based reporter technologies has pushed LFAs to better performance. These efforts have focused specifically on reporters made of organic fluorophores, quantum dots, lanthanide chelates, persistent luminescent phosphors, and upconversion phosphors. In most cases, photoluminescent reporters show enhanced sensitivity compared to conventional gold nanoparticle-based assays. Here, we examine the advantages and disadvantages of these different reporters and highlight their potential benefits in LFAs. Our assessment shows that photoluminescent-based LFAs can not only reach lower detection limits than LFAs with traditional reporters, but they also can be capable of quantitative and multiplex analyte detection. As a result, the photoluminescent reporters make LFAs well-suited for medical diagnostics, the food and agricultural industry, and environmental testing.
KW - lanthanide chelates
KW - lateral flow assay
KW - organic fluorophores
KW - persistent luminescent phosphors
KW - photoluminescent reporters
KW - quantum dots
KW - upconversion phosphors
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U2 - 10.1021/acsabm.1c01051
DO - 10.1021/acsabm.1c01051
M3 - Review article
C2 - 35014811
AN - SCOPUS:85122309840
VL - 5
SP - 82
EP - 96
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
SN - 2576-6422
IS - 1
ER -