Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements

E. Kellenbach; T. Härd; R. Boelens; K. Dahlman; J. Carlstedt-Duke; J. Å Gustafsson; G. A. van der Marel; J. H. van Boom; B. Maler; K. R. Yamamoto; R. Kaptein

doi:10.1007/BF01874574

Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements

E. Kellenbach, T. Härd, R. Boelens, K. Dahlman, J. Carlstedt-Duke, J. Å Gustafsson, G. A. van der Marel, J. H. van Boom, B. Maler, K. R. Yamamoto, R. Kaptein

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Abstract

Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr⁴⁵² and Tyr⁴⁷⁴ are polarised in both fragments while Tyr⁴⁹⁷ is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr⁴⁷⁴ polarization while the polarization of Tyr⁴⁵² is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr⁴⁷⁴ polarization is not due to protein-protein contacts but to interaction with DNA.

Original language	English (US)
Pages (from-to)	105-110
Number of pages	6
Journal	Journal of Biomolecular NMR
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/BF01874574
State	Published - May 1991

Keywords

Glucocorticoid receptor
Glucocortoid response elements
Photo-CIDNP
Protein-DNA interaction

ASJC Scopus subject areas

Spectroscopy
Biochemistry
General Biochemistry, Genetics and Molecular Biology

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Kellenbach, E., Härd, T., Boelens, R., Dahlman, K., Carlstedt-Duke, J., Gustafsson, J. Å., van der Marel, G. A., van Boom, J. H., Maler, B., Yamamoto, K. R., & Kaptein, R. (1991). Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements. Journal of Biomolecular NMR, 1(1), 105-110. https://doi.org/10.1007/BF01874574

Kellenbach, E, Härd, T, Boelens, R, Dahlman, K, Carlstedt-Duke, J, Gustafsson, JÅ, van der Marel, GA, van Boom, JH, Maler, B, Yamamoto, KR & Kaptein, R 1991, 'Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements', Journal of Biomolecular NMR, vol. 1, no. 1, pp. 105-110. https://doi.org/10.1007/BF01874574

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abstract = "Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA.",

keywords = "Glucocorticoid receptor, Glucocortoid response elements, Photo-CIDNP, Protein-DNA interaction",

author = "E. Kellenbach and T. H{\"a}rd and R. Boelens and K. Dahlman and J. Carlstedt-Duke and Gustafsson, \{J. {\AA}\} and \{van der Marel\}, \{G. A.\} and \{van Boom\}, \{J. H.\} and B. Maler and Yamamoto, \{K. R.\} and R. Kaptein",

year = "1991",

month = may,

doi = "10.1007/BF01874574",

language = "English (US)",

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pages = "105--110",

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AU - Kellenbach, E.

AU - Härd, T.

AU - Boelens, R.

AU - Dahlman, K.

AU - Carlstedt-Duke, J.

AU - Gustafsson, J. Å

AU - van der Marel, G. A.

AU - van Boom, J. H.

AU - Maler, B.

AU - Yamamoto, K. R.

AU - Kaptein, R.

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AB - Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA.

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KW - Glucocortoid response elements

KW - Photo-CIDNP

KW - Protein-DNA interaction

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Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements

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Keywords

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