Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements

E. Kellenbach, T. Härd, R. Boelens, K. Dahlman, J. Carlstedt-Duke, Jan-Ake Gustafsson, G. A. van der Marel, J. H. van Boom, B. Maler, K. R. Yamamoto, R. Kaptein

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalJournal of Biomolecular NMR
Volume1
Issue number1
DOIs
StatePublished - May 1 1991

Keywords

  • Glucocorticoid receptor
  • Glucocortoid response elements
  • Photo-CIDNP
  • Protein-DNA interaction

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

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