Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization

Chih Hang Anthony Tang, Shiun Chang, Adrienne W. Paton, James C. Paton, Dmitry I. Gabrilovich, Hidde L. Ploegh, Juan R. Del Valle, Chih Chi Andrew Hu

    Research output: Contribution to journalArticlepeer-review

    41 Scopus citations


    To relieve endoplasmic reticulum (ER) stress, IRE1 splices XBP1 messenger RNA (mRNA) or engages regulated IRE1- dependent decay (RIDD) of other mRNAs. Upon XBP1 deficiency, IRE1 switches to perform RIDD. We examined IRE1 in XBP1-deficient B cells and discovered that IRE1 undergoes phosphorylation at S729. We generated an anti-phospho-S729 antibody to investigate such phosphorylation. Compared with pharmacological ER stress inducers or Toll-like receptor ligands, the bacterial subtilase cytotoxin has an unusual capability in causing rapid and strong phosphorylation at S729 and triggering B cells to express spliced XBP1. To assess the function of S729 in IRE1, we generated S729A knock-in mice and found S729 is critically important for lipopolysaccharide-stimulated plasmablasts to respond to additional ER stress and for antibody production in response to immunization. We further crossed mice carrying an S729A mutation or ΔIRE1 (missing the kinase domain) with B cell-specific XBP1-deficient mice to trigger RIDD and discovered a critical role for S729 in regulating RIDD in B cells.

    Original languageEnglish (US)
    Pages (from-to)1739-1755
    Number of pages17
    JournalJournal of Cell Biology
    Issue number5
    StatePublished - May 1 2018

    ASJC Scopus subject areas

    • Cell Biology


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