TY - JOUR
T1 - Phospholipase D involvement in the plant oxidative burst
AU - Schroeder Taylor, Ann T.
AU - Low, Philip S.
N1 - Funding Information:
We thank Dr. S. Chandra and Dr. P. Heinstein for preparation of the Verticillium dahliae elicitor, Dr. S. Beer for the harpin elicitor, and Dr. X. Wang for phospholipase D antibodies. This work was supported by NSF Grant MCB 9303929.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997/8/8
Y1 - 1997/8/8
N2 - Pathogen-triggered generation of reactive oxidants, termed the oxidative burst, contributes to disease resistance in both plant and animal kingdoms. Since phospholipase D plays a key role in the neutrophil oxidative burst signaling cascade and is highly abundant in plants, we investigated its participation in the plant oxidative burst. Thin layer chromatography of extracted phospholipids revealed no changes in phosphatidic acid levels in soybean cells undergoing oxidant production, and no changes in phosphatidyl-ethanol biosynthesis could be detected when ethanol was present during elicitation. An inhibitor of phosphatidic acid hydrolase, propranolol, did not modify burst parameters or phosphatidic acid levels during the burst, suggesting our inability to detect phosphatidic acid accumulation was not due to rapid elimination. Furthermore, exogenous phosphatidic acid did not elicit a burst or enhance elicitor-stimulated bursts. Finally, ethanol, a substitute nucleophile, did not abrogate the burst. With data showing the presence of phospholipase D in soybean cells, these data argue that soybean phospholipase D does not participate in signaling the oxidative burst. This constitutes the first major difference between the plant and animal oxidative burst signal transduction pathways.
AB - Pathogen-triggered generation of reactive oxidants, termed the oxidative burst, contributes to disease resistance in both plant and animal kingdoms. Since phospholipase D plays a key role in the neutrophil oxidative burst signaling cascade and is highly abundant in plants, we investigated its participation in the plant oxidative burst. Thin layer chromatography of extracted phospholipids revealed no changes in phosphatidic acid levels in soybean cells undergoing oxidant production, and no changes in phosphatidyl-ethanol biosynthesis could be detected when ethanol was present during elicitation. An inhibitor of phosphatidic acid hydrolase, propranolol, did not modify burst parameters or phosphatidic acid levels during the burst, suggesting our inability to detect phosphatidic acid accumulation was not due to rapid elimination. Furthermore, exogenous phosphatidic acid did not elicit a burst or enhance elicitor-stimulated bursts. Finally, ethanol, a substitute nucleophile, did not abrogate the burst. With data showing the presence of phospholipase D in soybean cells, these data argue that soybean phospholipase D does not participate in signaling the oxidative burst. This constitutes the first major difference between the plant and animal oxidative burst signal transduction pathways.
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U2 - 10.1006/bbrc.1997.6965
DO - 10.1006/bbrc.1997.6965
M3 - Article
C2 - 9266820
AN - SCOPUS:0031559603
VL - 237
SP - 10
EP - 15
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -